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Epigenetic programming by trithorax supports the translation capacity of Drosophila memory neurons [RNA-seq Set1 & trr]

ABSTRACT: Epigenetic programming by trithorax supports the translation capacity of Drosophila memory neurons [RNA-seq Set1 & trr]

PROVIDER: PRJNA1117353 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29202214_1.fastq.gz	Fastqsanger.gz
	SRR29202214_2.fastq.gz	Fastqsanger.gz
	SRR29202215_1.fastq.gz	Fastqsanger.gz
	SRR29202215_2.fastq.gz	Fastqsanger.gz
	SRR29202216_1.fastq.gz	Fastqsanger.gz

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Epigenetic programming by trithorax supports the translation capacity of Drosophila memory neurons [RNA-seq Set1 & trr]

Project description:We have analyzed changes in histone H3 lysine 4 methylation (H3K4me) and gene expression resulting from trx knockdown (KD) in adult mushroom body neurons of Drosophila melanogaster.

2024-12-17 | GSE268425 | GEO

Distinct roles for COMPASS core subunits Set1, Trx, and Trr in the epigenetic regulation of Drosophila heart development

Project description:This study investigated the role Complex of Proteins Associated with Set1 (COMPASS) in heart development. These are evolutionarily highly conserved multiprotein complexes required for H3K4 methylation. In Drosophila, their core subunits are Set1, Trx, and Trr. We studied flies deficient for either of these three subunits their hearts. These flies showed high lethality at eclosion (emergence of adult flies from their pupal case) and significantly shortened lifespans for the adults that did emerge. Furthermore, their hearts showed reduced H3K4 monomethylation (H3K4me1) and dimethylation (H3K4me2), and significantly altered gene expression patterns. The data revealed that each of the COMPASS core subunits (Set1, Trx, and Trr) control methylation of different sets of genes with distinct temporal activity: Set1 throughout, whereas Trr was active early and Trx at later stages of heart development. We found that many metabolic pathways were active early in development and throughout, while muscle and heart differentiation processes were methylated during later stages of development. Taken together, the findings demonstrate the importance of COMPASS complexes during heart development, therewith supporting their implication in congenital heart diseases.

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Enhancer-associated H3K4 mono-methylation by Drosophila Trithorax-related, the Drosophila homolog of Mll3/Mll4

Project description:Mono-methylation of histone H3 on lysine 4 (H3K4me1) and acetylation of histone H3 on lysine 27 (H3K27ac) are histone modifications that are highly enriched over the body of actively transcribed genes and enhancers. Although in yeast all H3K4 methylation patterns including H3K4me1 are implemented by Set1/COMPASS, there are three classes of COMPASS-like complexes in Drosophila that could carry out H3K4me1 on enhancers: dSet1, Trithorax and Trithorax-related (Trr). Here, we report that Trr, the Drosophila homolog of mammalian Mll3/4, can function as a major H3K4 mono-methyltransferase on enhancers in vivo. Loss of Trr results in a global decrease of H3K4me1 and H3K27ac in various tissues. Assays with the cut wing margin enhancer imply a functional role for Trr in enhancer-mediated processes. A genome-wide analysis demonstrates that Trr is required for H3K4me1 and H3K27ac on chromatin signatures that resemble the histone modification patterns described for enhancers. Since Trr and mammalian Mll3/4 complexes are distinguished by bearing a unique subunit, the H3K27 demethylase UTX, we propose a model in which the H3K4 mono-methyltransferase Trr, and the H3K27 demethylase, UTX, cooperate to regulate the transition from inactive/poised to active enhancers. ChIP-seq of Trr, LPT, UTX in Drosophila S2 Cells. ChIP-seq of H3K4me1, H3K4me3, H3K27ac, H3K27me3 in WT and Trr knock-down Drosophila S2 cells. ChIP-seq of H3K4me1, H3K27me3 in LPT knock-down Drosophila S2 cells. ChIP-seq of LPT and UTX in Trr knock-down Drosophila S2 cells. ChIP-seq of H3K4me1 and H3K27me3 in MLL1(+/+), MLL1(-/-), MLL3(+/+), and MLL3(-/-) Mouse Embryonic Fibroblasts (MEFs).

2012-12-01 | E-GEOD-41440 | biostudies-arrayexpress

Epigenetic programming by trithorax supports the translation capacity of Drosophila memory neurons.

Project description:Epigenetic programming by trithorax supports the translation capacity of Drosophila memory neurons.

| PRJNA999446 | ENA

Enhancer-associated H3K4 mono-methylation by Drosophila Trithorax-related, the Drosophila homolog of Mll3/Mll4

2012-12-01 | GSE41440 | GEO

Epigenetic programming by trithorax supports the translation capacity of Drosophila memory neurons [ChIP-seq]

Project description:Epigenetic programming by trithorax supports the translation capacity of Drosophila memory neurons [ChIP-seq]

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Epigenetic programming by trithorax supports the translation capacity of Drosophila memory neurons [RNA-seq trx]

Project description:Epigenetic programming by trithorax supports the translation capacity of Drosophila memory neurons [RNA-seq trx]

| PRJNA999448 | ENA