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A Spatiotemporal Transcriptome Revealed Stalk Development in Pearl Millet

ABSTRACT: A Spatiotemporal Transcriptome Revealed Stalk Development in Pearl Millet

PROVIDER: PRJNA1119429 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29263744_1.fastq.gz	Fastqsanger.gz
	SRR29263744_2.fastq.gz	Fastqsanger.gz
	SRR29263745_1.fastq.gz	Fastqsanger.gz
	SRR29263745_2.fastq.gz	Fastqsanger.gz
	SRR29263746_1.fastq.gz	Fastqsanger.gz

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A Spatiotemporal Transcriptome Revealed Stalk Development in Pearl Millet

Project description:Pearl millet is a major cereal crop that feeds more than 90 million people worldwide in arid and semi-arid regions. The stalk phenotypes of Poaceous grasses are critical for their productivity and stress tolerance, however, the molecular mechanisms governing stalk development in pearl millet remained to be deciphered. In this study, we spatiotemporally measured 19 transcriptomes for stalk internodes of four different early developmental stages. Data analysis of the transcriptomes defined 4 developmental zones on the stalks and identified 12 specific gene sets with specific expression patterns across the zones. Using weighted gene co-expression network analysis (WGCNA), we found that 2 co-expression modules together with candidate genes were involved in stalk elongation and thickening of pearl millet. Among the elongation-related candidate genes, we established by SELEX that a MYB-family transcription factor PMF7G02448 can bind to the promoters of three cell wall synthases genes (CesAs). In summary, these findings provide insights into stalk development and offer potential targets for future genetic improvement of pearl millet.

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Pearl millet seedlings infected with rust, or treated with MeJA or SA

Project description:This SuperSeries is composed of the following subset Series: GSE13478: Pearl millet seedlings treated with methyl jasmonate (MeJA) GSE13479: Pearl millet seedlings infected with rust (Puccinia substriata) GSE13480: Pearl millet seedlings treated with salicylic acid (SA) Refer to individual Series

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Project description:Transcriptional profiling of rust-infected pearl millet seedlings over time [0h, 20h, 5d and 8d post infection (pi)]. Keywords: Time course, Stress response Loop design. All time points compared with time = 0 h in data analysis. Two biological replicates per sample, and one technical dye swap replicate.

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