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Inducible keratinocyte-specific Mettl3-deficient mice develop spontaneous skin lesions

ABSTRACT: Inducible keratinocyte-specific Mettl3-deficient mice develop spontaneous skin lesions

PROVIDER: PRJNA1123710 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29405258_1.fastq.gz	Fastqsanger.gz
	SRR29405258_2.fastq.gz	Fastqsanger.gz
	SRR29405259_1.fastq.gz	Fastqsanger.gz
	SRR29405259_2.fastq.gz	Fastqsanger.gz
	SRR29405260_1.fastq.gz	Fastqsanger.gz

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Inducible keratinocyte-specific Mettl3-deficient mice develop spontaneous skin lesions

Project description:We conducted RNA sequencing analysis on skin tissues obtained from spontaneous inflammatory skin lesions in keratinocyte-specific Mettl3 knockout (K14CreERT2;Mettl3fl/fl) mice and their controls (Mettl3fl/fl). In brief, we observed spontaneous skin inflammation in the tail of K14CreERT2;Mettl3fl/fl mice 2-3 weeks following tamoxifen injection. The objective of this study was to investigate the impact and underlying mechanism of Mettl3 in epidermal keratinocytes regarding skin inflammation

2024-08-20 | GSE269796 | GEO

Transcription profiling of A. thaliana acd2-2 mutants leaves with spontaneous lesions vs. leaves without lesions

Project description:Arabidopsis acd2-2 mutant was used. Leaves conataining spontaneous lesions were compared to leaves without lesions

2006-12-04 | E-MEXP-912 | biostudies-arrayexpress

Mettl3 deficiency in epidermal keratinocytes exacerbates psoriasis-like inflammation [MeRIPseq_mice]

Project description:We analyzed epidermal samples from keratinocyte-specific knockout Mettl3 (K14CreERT2;Mettl3fl/fl) mice and their controls (Mettl3fl/fl) in imiquimod-induced psoriasis-like skin lesions. Briefly, we established an imiquimod-induced psoriasis model in K14CreERT2;Mettl3fl/fl mice and their controls after tamoxifen injection, and epidermal samples were separated and collected from the skin lesions of the mice after 3 or 6 days of modeling (n=3 per group). The aim of this study was to investigate the mechanism by which Mettl3-mediated epidermal m6A methylation affects skin inflammation.

2024-08-20 | GSE268745 | GEO

Spontaneous Endometritotic Lesions Compared to Eutopic Endometrium in the Baboon

Project description:The development and progression of endometriotic lesions are poorly understood, but immune cell dysfunction and inflammation are closely associated with the pathophysiology of endometriosis. A lack of suitable 3D in vitro models permitting the study of interactions between cell types and the microenvironment is a contributing factor. To address this limitation, we developed endometriotic organoids (EO) to explore the role of epithelial-stromal interactions and model peritoneal cell invasion associated with lesion development (GSE202661). These organoids were compared to spontaneous endometriotic lesions from baboons (Papio anubis).

2022-06-15 | GSE202729 | GEO

Spontaneous Endometritotic Lesions Compared to Eutopic Endometrium in the Baboon

Project description:Spontaneous Endometritotic Lesions Compared to Eutopic Endometrium in the Baboon

| PRJNA837178 | ENA

METTL3 promotes translation in human cancer cells

Project description:we find METTL3 associates with polyribosomes and promotes translation. METTL3 depletion inhibits translation, and both wild-type and catalytically inactive METTL3 promote translation when tethered to the 3' untranslated region (UTR) of a reporter mRNA. Mechanistically, METTL3 enhances mRNA translation through an interaction with the translation initiation machinery. m6A seq in A549 and H1299 cells, RNA seq in METTL3 knockdown cells

2016-07-03 | E-GEOD-76367 | biostudies-arrayexpress

Rab11FIP1-Deficient Mice Develop Spontaneous Inflammation and Show Increased Susceptibility to Colon Damage

Project description:Rab11FIP1-Deficient Mice Develop Spontaneous Inflammation and Show Increased Susceptibility to Colon Damage

| PRJNA826912 | ENA

Expression data from keratinocyte-specific Zfp36-deficient mouse skin treated with imiquimod

Project description:Tristetraprolin (TTP, encoded by Zfp36) regulates the mRNA stability of several important cytokines. Due to the critical role of this RNA-binding protein in the control of inflammation, TTP deficiency leads to the spontaneous development of a complex inflammatory syndrome. So far, this phenotype has been largely attributed to dysregulated production of TNF and IL-23 by myeloid cells such as macrophages or dendritic cells. Here, we generated mice with conditional deletion of TTP in keratinocytes. These mice developed exacerbated inflammation in the imiquimod-induced psoriasis model. Furthermore, these mice progressively developed a spontaneous pathology with systemic inflammation, psoriatic-like skin lesions and dactylitis. Finally, we provide evidence that keratinocyte-derived TNF productin drives the different pathological features. In summary, these findings expand current views on the initiation of psoriasis and related arthritis by revealing the keratinocyte-intrinsic role of TTP.

2017-06-14 | GSE90450 | GEO

METTL3 KD in HepG2 cells

Project description:To gain insight into possible processes that require m6A for their function, METTL3 was knocked down (KD) in HepG2 cells by siRNA transfections Differential expression analysis of METTL3 KD versus mock-transfected HepG2 cells, in 2 biological replicates

2012-04-25 | E-GEOD-37001 | biostudies-arrayexpress

Binding motif analysis of METTL3，WTAP in METTL3，WTAP overexpressed Human 293T cells

Project description:label the cells overexpressed Myc tagged METTL3 and Flag tagged WTAP with 4-SU, the RNA bound by METT3,WTAP can be got by Myc or Flag IP followed by RNA isolation by using the TRIzol (Invitrogen) reagent by following the company manual.the RNA was then used for library preparation using a TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol.The libraries were sequenced using HiSeq2000 (Illumina) in single-read mode, creating reads with a length of 50 bp. Sequencing chemistry v2 (Illumina) was used and samples were multiplexed in two samples per lane. discovery of the binding motif of METTL3，WTAP in METTL3，WTAP overexpressed Human 293T cells

2014-01-16 | E-GEOD-50580 | biostudies-arrayexpress

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