Project description:This SuperSeries is composed of the following subset Series: GSE9930: Wild type 3 hour exposure to zymolyase GSE9931: hog1 mutant 3 hour exposure to zymolyase GSE9932: slt2 mutant 3 hour exposure to zymolyase GSE9933: rlm1 mutant 3 hour exposure to zymolyase GSE9934: msn2/4 mutant 3 hour exposure to zymolyase S. cerevisiae wild type (BY4741) and mutants were grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with 5units/ml Zymolyase 100T. Cells were collected at 3 hours of growth, frozen at -80 °C and processed for RNA extraction. The total RNA from one culture was analyzed, and, in addition, for this sample two different hybridizations were performed, including fluorochrome swapping in order to minimize transcriptional changes due to technical variability. This therefore corresponded to two DNA microarrays analyzed.
Project description:We did transcription profiling on the effect of rlm1 (MAPK Slt2 transcription factor) deletion, slt2 (MAPK of Cell wall intregity pathway) deletion, bcy1 (Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA)) deletion and msn2/4 (Stress-responsive transcriptional activators) deletion in genes involved in Caspofungin response (2 hours of treatment). In addition, we analyzed the genome-wide expression profile of the wild type strain in response to Aminocandin (2 hours of treatment).
Project description:In our previous work, we had found that Saccharomyces cerevisiae needs of the Hog1 and Slt2 proteins to growth in a low pH environment caused by sulfuric acid, one of the stress factors during the process of ethanol production. Then was performed the gene-wide expression analysis in the hog1∆ and slt2∆ mutants in order to reveal the function of the Hog1p and Slt2p MAP Kinases in the regulation of S. cerevisiae global gene expression upon stress by sulfuric acid.
Project description:Expression data from yeast exposed to Caspofungin (wild type strain and rlm1, slt2, msn2/4 and bcy1 mutants) and Aminocandin (wild type strain)
Project description:A series of experiments comparing the gene expression response before and after 30 min 0.7M NaCl exposure in WT and mutant yeast strains. Mutant strains were identified as having a defect in acquiring resistance to H2O2 following mild NaCl pretreatment. The mutants in this study were taken from the Yeast Knock-Out Collection, mat a. Unstressed cells vs cells exposed to 0.7M NaCl for 30 min; 5 replicates for WT, 3 replicates for hog1∆, msn2∆, mck1∆, pde2∆, rim101∆, and 2 replicates for the remaining mutants.
Project description:We did transcription profiling on the effect of rlm1 (MAPK Slt2 transcription factor) deletion and swi3 (component of SWI/SNF complex involved in chromatin remodeling) deletion in genes involved in cell wall stress (Congo Red) response.
Project description:We did transcription profiling on the effect of rlm1 deletion, gene involved in cell wall stress response Keywords: cell wall stress response S. cerevisiae (rlm1 mutant) was grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with 5units/ml Zymolyase 100T. Cells were collected at 3 hours of growth, frozen at -80 °C and processed for RNA extraction. The total RNA from two different cultures was analyzed, and, in addition, for each sample two different hybridizations were performed, including fluorochrome swapping in order to minimize transcriptional changes due to technical variability. This therefore corresponded to four DNA microarrays analyzed for each condition.
