Project description:The recent release of a large number of genomes from ectomycorrhizal, orchid mycorrhizal and root endophytic fungi have provided deep insight into fungal lifestyle-associated genomic adaptation. Comparative analyses of symbiotic fungal taxa showed that similar outcomes of interactions in distant related root symbioses are examples of convergent evolution. The order Sebacinales represents a sister group to the Agaricomycetes (Basidiomycota) that is comprised of ectomycorrhizal, ericoid-, orchid- mycorrhizal, root endophytic fungi and saprotrophs (Oberwinkler et al., 2013). Sebacinoid taxa are widely distributed from arctic to temperate to tropical ecosystems and are among the most common and species-rich groups of ECM, OM and endophytic fungi (Tedersoo et al., 2012, Tedersoo et al., 2010, Oberwinkler et al., 2013). The root endophyte Piriformospora indica and the orchid mycorrhizal fungus S. vermifera (MAFF 305830) are non-obligate root symbionts which were shown to be able to interact with many different experimental hosts, including the non-mycorrhizal plant Arabidopsis thaliana. These two fungi display similar colonization strategies in barley and in Arabidopsis and the ability to establish beneficial interactions with different hosts (Deshmukh et al., 2006). Colonization of the roots by P. indica and S. vermifera results in enhanced seed germination and biomass production as well as increased resistance against biotic and abiotic stresses in its experimental hosts, including various members of the Brassicaceae family, barley, Nicotiana attenuata and switchgrass (Ghimire, 2011, Ghimire et al., 2009, Ghimire et al., 2011, Waller et al., 2008, Barazani et al., 2007, Deshmukh et al., 2006). Microarray experiments were performed to identify and characterize conserved sebacinoid genes as key determinants in the Sebacinales symbioses.

2015-06-01 | GSE60736 | GEO

Switchgrass root endophytic diazotrophs

Project description:Switchgrass root endophytic diazotrophs Raw sequence reads

| PRJNA560210 | ENA

Proteomic analysis of sap reveals root-shoot communication dynamics in potato and barley following flg22 treatment

Project description:Potato (Solanum tuberosum, variety Princ) and barley (Hordeum vulgare, variety Sebastian) plants were cultivated in a controlled environment. Plants were grown in Potgrond H soil (Klasmann-Deilmann GmbH, Germany) under a 12-hour photoperiod with a constant temperature of 21 °C and a photon flux density of 100 μmol m⁻² s⁻¹. Potato tubers were used as starting material, while barley plants were grown from surface-sterilized seeds. After six weeks of cultivation for potato and three weeks for barley, the sap proteome was collected by cutting the stems 10–20 mm above the soil surface. Root exudates were sampled into three fractions over a two-hour period: F1 (15 min), F2 (75 min), and F3 (135 min). Each experiment included at least three independent biological replicates. A subset of plants was pre-incubated with flg22, a conserved peptide sequence derived from bacterial flagellin (QRLSTGSRINSAKDDAAGLQIA, >95% purity; ProteoGenix, France). Flg22 solution (1 μM flg22, 0.025% v/v Silwet L-77) was applied by spraying the leaves and pouring the solution under the pot. Potato plants received 30 ml and 200 ml of the solution for spraying and watering, respectively. Due to their smaller size, barley plants were treated with half the solution volume. Mock-treated plants received a solution containing only 0.025% Silwet L-77. After 24 hours, plants were cut, and the third fraction of the sap (75-135 min) was collected. Root and shoot tissues were collected in liquid nitrogen, the sap was collected as described above. Each experiment included at least three independent biological replicates.

2024-07-20 | PXD053406 | Pride

Coffea endophytic microbiome

Project description:Coffea endophytic microbiome Raw sequence reads

| PRJNA903488 | ENA

Test of submission

Project description:Test of submission

| PRJEB30876 | ENA

Project description:Root Endophytic Microbiome

| PRJNA565911 | ENA