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ABSTRACT: sciMET-ATAC: simultaneous DNA methylation and chromatin accessibility analysis of single cells

PROVIDER: PRJNA1134352 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR29772078_1.fastq.gz	Fastqsanger.gz
	SRR29772078_2.fastq.gz	Fastqsanger.gz
	SRR29772079_1.fastq.gz	Fastqsanger.gz
	SRR29772079_2.fastq.gz	Fastqsanger.gz

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Project description:Here we present Perturb-ATAC, a method which combines multiplexed CRISPR interference or knockout with genome-wide chromatin accessibility profiling in single cells, based on the simultaneous detection of CRISPR guide RNAs and open chromatin sites by Assay of Transposase-accessible Chromatin with sequencing (ATAC-seq). We applied Perturb-ATAC to transcription factors (TFs), chromatin-modifying factors, and noncoding RNAs (ncRNAs) in ~3,700 single cells, encompassing more than 75 unique genotype-phenotype relationships.

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