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Arnica montana strain:domestic

ABSTRACT: Arnica montana strain:domestic Transcriptome or Gene expression

PROVIDER: PRJNA233448 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1150769_1.fastq.gz	Fastqsanger.gz
	SRR1150769_2.fastq.gz	Fastqsanger.gz

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Arnica montana

Project description:Arnica montana overview

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Arnica montana stimulates extracellular matrix gene expression in human macrophages differentiated to wound-healing phenotype.

Project description:Arnica m. effects were associated with a purported anti-inflammatory and tissue healing actions after trauma, bruises, or tissue injuries, but its cellular and molecular mechanisms are largely unknown. Here Arnica m. dilutions were tested using an in vitro model of macrophages polarized towards a “wound-healing” phenotype. The monocyte-macrophage human THP-1 cell line was cultured and differentiated with phorbol-myristate acetate and Interleukin-4, then exposed for 24 h to Arnica m. centesimal (c) dilutions 2c, 3c, 5c,9c, 15c or Control. None of these treatments affected cell viability. A total of 20 genes were differentially expressed comparing cells treated with Arnica m. 2c with those treated with Control only. Of these, 7 genes were up-regulated and 13 were down-regulated. Functional gene enrichment analysis showed that the most significantly upregulated function concerned 4 genes with a conserved site of EGF-like region (p<0.001) and three genes of proteinaceous extracellular matrix, including heparin sulphate proteoglycan 2 (HSPG2), fibrillin 2 (FBN2), and fibronectin (FN1) (p <0.01). Protein assay in supernatants confirmed a statistically significant increase of fibronectin production in Arnica m. 2c treated cells (p<0.05). Pooled extracts of cells treated with increasing dilutions of Arnica m. (3c, 5c, 15c) showed up-regulation of the same group of genes although with lower effect size. The down-regulated transcripts derive from mitochondrial genes coding for some components of electron transport chain. These findings provide new insights into the action of Arnica m. in tissue healing and repair, identifying increased fibronectin production by macrophages as a major therapeutic target.

2016-11-17 | GSE77382 | GEO

Arnica montana stimulates extracellular matrix gene expression in human macrophages differentiated to wound-healing phenotype. Tested on 5 concentrations.

2016-11-17 | GSE77381 | GEO

Arnica chamissonis

Project description:Arnica chamissonis Transcriptome or Gene expression

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Project description:Arnica mallotopus overview

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Drosophila montana

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Helianthus annuus strain:domestic

Project description:Helianthus annuus strain:domestic Transcriptome or Gene expression

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Gene expression in Drosophila montana

Project description:We created a custom-made microarray for D. montana with 101 genes known to affect traits important in diapause, photoperiodism, reproductive behaviour, circadian clock and stress tolerance in model Drosophila species. This array gave us a chance to filter out genes showing expression changes during photoperiodic reproductive diapause in a species adapted to live in northern latitudes with high seasonal changes.

2010-03-04 | GSE18475 | GEO

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