Project description:We had three goals to perform this study: compare normal mice and colitis mice，compare berberine effect in normal mice and compare berberine effect in colitis mice
2022-11-26 | GSE197342 | GEO
Project description:Effect of amylin on intestinal microflora in mice
| PRJNA694540 | ENA
Project description:Intestinal microflora of mice
| PRJNA862196 | ENA
Project description:Effect of METH treatment on intestinal microflora in mice
| PRJNA926793 | ENA
Project description:Effect of Holothuria leucospilota on the intestinal microflora of mice
Project description:Commensal microbiota contribute to gut homeostasis and influence gene expression. Intestinal organoid culture closely represent intestinal epithelium and retain intestinal stem cells and dynamic recovery capabilities as well as all major cell types of the intestinal epithelium. We established organoid culture using colon crypts isolated from germ-free (GF), and gnotobiotic mice monocolonized either with the E.coli strain O6K13 (O) or Nissle 1917 strain (N). The expression profiles of these organoids were compared to the organoid culture isolated from conventionally reared (CR) mice in order to disclose genes differentially expressed in response to the change in the intestinal microflora composition.
Project description:This study delineated how small intestinal resident microflora impact gene expression in Paneth cells. Experiment Overall Design: Paneth cells were isolated by laser capture microdissection from the small intestines of germ-free and conventionalized (10 day) mice. RNAs from 3 mice per group were pooled, and duplicate RNAs from each group were amplified and hybridized to Affymetrix arrays.
Project description:This study delineated how small intestinal resident microflora impact gene expression in Paneth cells. Keywords: functional genomics; transcriptional profiling
