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ABSTRACT: Plant sample from Vitis vinifera

PROVIDER: PRJNA1140405 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR30009869_1.fastq.gz	Fastqsanger.gz
	SRR30009869_2.fastq.gz	Fastqsanger.gz
	SRR30009870_1.fastq.gz	Fastqsanger.gz
	SRR30009870_2.fastq.gz	Fastqsanger.gz
	SRR30009871_1.fastq.gz	Fastqsanger.gz

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Project description:Vitis vinifera RNA degradome Isolated polyadenylated RNA from total RNA extracts of Vitis vinifera leaves, were ligated to 5'-adapter that include san MmeI recognition site. The ligated products were purified again, reverse transcribed and cleaved with MmeI. The 5' fragments were purified from gel and to a 3'- dsDNA adapter and PCR amplified. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

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Project description:Methods:transcriptomes of the different development stages of Vitis vinifera cv. Cabernet Sauvignon and Vitis quinquangularis accession Danfeng-2 were analyzed using Illumina Hiseq 2500. The sequence reads that passed quality filters were analyzed: TopHat followed by Cufflinks. mRNA profiles of different development stages of Vitis vinifera cv. Cabernet Sauvignon and Vitis quinquangularis accession Danfeng-2 were generated by deep sequencing, in triplicate, using Illumina Hiseq 2500.

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