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ABSTRACT: Exome capture optimization of Pinus sylvestris

PROVIDER: PRJNA1145653 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR30170691_1.fastq.gz	Fastqsanger.gz
	SRR30170691_2.fastq.gz	Fastqsanger.gz
	SRR30170692_1.fastq.gz	Fastqsanger.gz
	SRR30170692_2.fastq.gz	Fastqsanger.gz
	SRR30170693_1.fastq.gz	Fastqsanger.gz

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EVOTREE: What makes a tree a tree? Characterization and evolution of the gene regulatory network underlying wood formation: Pinus sylvestris

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Scots pine (Pinus sylvestris) differentially interacts with Cenococcum geophilum and other simultaneously inoculated mycorrhizal (ECM) fungi

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Project description:Embryos and megagametophytes samples belonging to four early developmental stages during seed development in Pinus sylvestris were collected from a seed orchard in central Sweden. Dominant (DO) and subordinate (SU) zygotic embryos were collected separately. RNA was isolated from this samples and the corresponding cDNA synthesized and subjected to RNA sequencing (one biological replicate). In total nine RNA-seq libraries were constructed, five for embryo samples (E1, E2, E3DO, E3SU, E4) and four for megagametophytes (M1, M2, M3 and M4). qRT-PCR analysis have been done to validate the RNA-seq expression results.

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