Project description:This SuperSeries is composed of the following subset Series: GSE13858: Global survey of miRNA microarray of uterus, ovariectomized female mice with or without estrogen (E2) treatment GSE13859: Global survey of miRNA microarray of whole embryo, wild type vs estrogen receptor alpha knockout mice Refer to individual Series
Project description:To examine the effect of estrogen receptor alpha for the miRNA expression, total RNA extracted from whole embryos at E18.5 of wild type and Estrogen Receptor alpha knock-out mice
Project description:To examine the effect of estrogen receptor alpha for the miRNA expression, total RNA extracted from whole embryos at E18.5 of wild type and Estrogen Receptor alpha knock-out mice
Project description:To examine the effect of estrogen receptor alpha for the miRNA expression, total RNA extracted from whole embryos at E18.5 of wild type and Estrogen Receptor alpha knock-out mice Two group experiment (wild type and ER alpha KO)
Project description:To examine the effect of estrogen receptor alpha for the miRNA expression, total RNA extracted from whole embryos at E18.5 of wild type and Estrogen Receptor alpha knock-out mice Two group experiment (wild type and ER alpha KO)
Project description:Estrogen-related receptor (ERR) alpha is an orphan nuclear receptor highly expressed in the kidneys. ERRalpha is implicated in renal sodium and potassium homeostasis and blood pressure regulation. We used microarray analysis to identify differentially expressed genes in ERR alpha knockout mice kidneys versus wild-type. The results provide insight on the roles of ERRalpha in the kidney.
Project description:We have previously shown that total estrogen receptor alpha (ERalpha knockout (KO) mice exhibit hepatic insulin resistance. To investigate the contribution of hepatic ERalpha action for the observed phenotype, we established a liver-selective ERalphaKO mouse model, LERKO. We demonstrate that LERKO mice have efficient reduction of ERalpha selectively within the liver. However, LERKO and wild type control mice do not differ in body weight, and have a comparable hormone profile as well as insulin and glucose response, even when challenged with a high fat diet. Furthermore, LERKO mice display very minor changes in their hepatic transcript profile. Collectively, our findings indicate that hepatic ERalpha action may not be the initiating factor for the previously identified hepatic insulin resistance in ERalphaKO mice. We have previously shown that total estrogen receptor alpha (ERalpha knockout (KO) mice exhibit hepatic insulin resistance. To investigate the contribution of hepatic ERalpha action for the observed phenotype, we established a liver-selective ERalphaKO mouse model, LERKO. Using microarray analysis, we compared the hepatic transcriptional profile of LERKO vs control mice.
