Extrauterine support of preterm lambs achieves similar transcriptomic profiling to late pre-term lamb brains
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ABSTRACT:
PROVIDER: PRJNA1149839 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR30299600_1.fastq.gz | Fastqsanger.gz | |||
| SRR30299600_2.fastq.gz | Fastqsanger.gz | |||
| SRR30299601_1.fastq.gz | Fastqsanger.gz | |||
| SRR30299601_2.fastq.gz | Fastqsanger.gz |
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Project description:Purpose: The goal of this study was to measure by RNA-seq the impact of preterm birth and invasive mechanical ventilation verses noninvasive respiratory support on the lung transcriptome Methods: Lung mRNA profiles were generated for: 1) Unventilated preterm lamb, 2) preterm lambs delivered at gd131 and intubated and mechanically ventilated for 3 days, 3) preterm lambs delivered at gd131 and not intubated and resuscitated by placing a face mask over the nose and mouth and controlling O2 delivery via a computer-controlled electronic blower device, 4) unventilated naturally delivered full-term lambs. Results: Using an optimized data analysis workflow, we mapped between 61 and 81 million sequence reads per sample to the sheep genome Oar_v3.1/oviAri3 Conclusions: Our study represents the first detailed analysis of ventilated preterm lung transcriptomes, with biologic replicates, generated by RNA-seq
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