Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis of human embryonic stem cell-derived cartilage pellets at three stages of differentiation

ABSTRACT: Transcriptome analysis of human embryonic stem cell-derived cartilage pellets at three stages of differentiation

PROVIDER: PRJNA1153038 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR30420901_1.fastq.gz	Fastqsanger.gz
	SRR30420901_2.fastq.gz	Fastqsanger.gz
	SRR30420902_1.fastq.gz	Fastqsanger.gz
	SRR30420902_2.fastq.gz	Fastqsanger.gz
	SRR30420903_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 28

Similar Datasets

Transcriptome analysis of human embryonic stem cell-derived cartilage pellets at three stages of differentiation

Project description:RNA-sequencing data was obtained from cultured cartilage pellets differentiated from human embryonic stem cells via neural crest intermediate at three consecutive stages of differentiation. The transcriptome of each stage was analyzed and compared against other stages, creating a "timeline" of cartilage differentiation.

2024-11-12 | GSE275825 | GEO

Three life stages

Project description:Three life stages

| PRJNA589548 | ENA

Expression data from growth plate cartilage of control and immobilized embryonic chickens

Project description:Affymetrix Chicken Gene 1.0 ST Array (ChiGene-1_1-st) profiles were generated from growth plate cartilage derived from the distal femur and proximal tibia of embryonic chickens at three developmental stages (day 12, 13 and 15) corresponding to stages prior to and immediately after the acqusition of mechano-responsive cartilage growth in the femur but not tibia (which does not respond to mechanical stimuli until later stages). Additionally, array profiles were generated from growth plate cartilage from the distal femur and proximal tibia of E15 chicks which were pharmacologically immobilized by decamethonium bromide treatment, which reduces longitudinal growth of the femur only at this stage.

2016-12-31 | GSE77412 | GEO

Pre-transplantational control of the post-transplantational fate of human pluripotent stem cell-derived cartilage

Project description:Cartilage pellets generated from ectomesenchymal progeny of human pluripotent stem cells (hPSCs) in vitro eventually show signs of commitment of chondrocytes to hypertrophic differentiation. When transplanted subcutaneously, most of the surviving pellets were fully mineralized by 8 weeks. In contrast, treatment with the adenylyl cyclase activator, forskolin, in vitro resulted in slightly enlarged cartilage pellets containing an increased proportion of proliferating immature chondrocytes that expressed very low levels of hypertrophic/terminally matured chondrocyte-specific genes. Forskolin treatment also enhanced hyaline cartilage formation by reducing type I collagen gene expression and increasing sulfated glycosaminoglycan accumulation in the developed cartilage. Furthermore, forskolin treatment in vitro increased the frequency at which the cartilage pellets maintained unmineralized chondrocytes after subcutaneous transplantation. To elucidate the type of cartilage that forskolin preferentially promotes and potential molecular mechanisms involved in the forskolin-suppression of chondrocyte hypertrophic differentiation in vitro, we performed genome-wide, comparative transcriptomic analyses on the cartilage pellets formed from 3 to 4 independent pellet cultures of ectomesenchymal cells with or without forskolin treatment. RNAs were isolated on day 26-28 and mRNA-sequencing (RNA-seq) analyses were performed.

2020-06-01 | GSE116173 | GEO

Transcriptome analysis of ear cartilage lipochondrocytes at multiple stages of differentiation.

Project description:RNA-seq data was obtained from mouse ear cartilage lipochondrocytes at varying postnatal stages. The transcriptome of each stage was analyzed and compared against other stages, creating a "timeline" of cellular events. Adult transcriptome data was analyzed and compared against adult white adipose tissue adipocyte transcriptome.

2024-11-12 | GSE116119 | GEO

Expression data from the rat knee articular cartilage at different developmental stages

Project description:Age as the primary rise factor could be play an important role in incidence and development of osteoarthritis. Several studies have confirmed some tissue specific microRNA were associated with development of osteoarthritis. But if age related microRNA or miRNA cluster would be involved in pivotal post-transcriptional gene regulation in osteoarthritis is unclear. In view of this, we have an idea that several age-related miRNAs would be screened from the rat knee cartilage at different development ages by miRNAs Microarray analysis. We used microarrays to detail the global programme of gene expression underlying the rat knee cartilage and identified distinct classes of age-related miRNAs during this process. The rat knee articular cartilage were selected at successive stages of the rat developmental for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of cartilage at each developmental stage in order to increase the temporal resolution of expression profiles. To that end, we hand-selected cartilage according to the rat developmental stages, i.e. seven time-points: newborn (T0), childhood (T1), youth(T2), adult (T3), middle-aged (T4) early-stage elderly(T5) and latter-stage elderly(T6). The objective of the study is to identify miRNA profile of knee articular cartilage at different developmental ages in rats. Total RNA were extracted from the knee articular cartilage of Sprague-Dawley rats at postnatal day 0(T0), week1(T1), week 4(T2), mon3(T3), mon 6(T4), mon 12(T5), and mon 18(T6). The microRNA profile in the specimens was detected with the Affymetrix GeneChipÂ® miRNA 3.0 Array.

2013-06-26 | E-GEOD-48266 | biostudies-arrayexpress

Equine Embryonic Interzone and of Articular Cartilage Zones

Project description:Cells from the superficial (AACS), middle (AACM) and deep (AACD) adult articular cartilage zones and the intermediate (II) and outer (OI) interzone layers and the transient embryonic cartilage of the long bone anlagen (EC) at gestational day 40 were separately collected using laser capture microdissection and microarray analysis was performed to confirm appropriate layer selection.

2019-06-01 | GSE83273 | GEO

Expression data of LncRNA from the rat (Sprague-Dawley) knee articular cartilage at different developmental stages

Project description:Age as the primary rise factor could be play an important role in incidence and development of osteoarthritis. A few studies have confirmed some tissue specific lncRNA were associated with development of osteoarthritis. But if age related lncRNA would be involved in pivotal post-transcriptional gene regulation in osteoarthritis is unclear. In view of this, we have an idea that several age-related lncRNA would be screened from the rat knee cartilage at different development ages by lncRNAs Microarray analysis. We used microarrays to detail the global programme of gene expression underlying the rat knee cartilage and identified distinct classes of age-related lncRNA during this process. The rat knee articular cartilage were selected at successive stages of the rat developmental for RNA extraction and hybridization on Affymetrix lncRNA arrays. We sought to obtain homogeneous populations of cartilage at each developmental stage in order to increase the temporal resolution of expression profiles. To that end, we hand-selected cartilage according to the rat developmental stages, i.e. seven time-points: newborn (T0), youth(T1), adult (T2), early-stage elderly(T3) and latter-stage elderly(T4).

2015-03-05 | E-GEOD-66554 | biostudies-arrayexpress

RNAseq of maize stem at three developmental stages

Project description:RNAseq of maize stem at three developmental stages

| PRJNA476069 | ENA

Transcriptome analysis of ear cartilage lipochondrocytes at multiple stages of differentiation.

Project description:Transcriptome analysis of ear cartilage lipochondrocytes at multiple stages of differentiation.

| PRJNA477329 | ENA