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The influence of microRNA-455-3p on gene expression in the developing chick limb bud

ABSTRACT: The influence of microRNA-455-3p on gene expression in the developing chick limb bud

PROVIDER: PRJNA1157752 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30568778_1.fastq.gz	Fastqsanger.gz
	SRR30568778_2.fastq.gz	Fastqsanger.gz
	SRR30568779_1.fastq.gz	Fastqsanger.gz
	SRR30568779_2.fastq.gz	Fastqsanger.gz
	SRR30568780_1.fastq.gz	Fastqsanger.gz

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The influence of microRNA-455-3p on gene expression in the developing chick limb bud

Project description:MicroRNA-455 is a key microRNA in cartilage, strongly induced by Sox9. Overexpression of miR-455-3p in the developing chick limb bud at HH18-19 causes a reduction in limb bud size at HH24-25.

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Long non-coding RNA HOTTIP promotes CCL3 expression and induces cartilage degradation by sponging miR-455-3p

Project description:Long non-coding RNAs (lncRNAs) play pivotal roles in diseases such as osteoarthritis (OA). However, knowledge of the biological roles of lncRNAs is limited in OA. We aimed to explore the biological function and molecular mechanism of HOTTIP in chondrogenesis and cartilage degradation. We used the human mesenchymal stem cell (MSC) model of chondrogenesis, in parallel with, tissue biopsies from normal and OA cartilage to detect HOTTIP, CCL3, and miR-455-3p expression in vitro. Biological interactions between HOTTIP and miR-455-3p were determined by RNA silencing and overexpression in vitro. We evaluated the effect of HOTTIP on chondrogenesis and degeneration, and its regulation of miR-455-3p via competing endogenous RNA (ceRNA). Our in vitro ceRNA findings were further confirmed within animal models in vivo. Mechanisms of ceRNAs were determined by bioinformatic analysis, a luciferase reporter system, RNA pull-down, and RNA immunoprecipitation (RIP) assays. We found reduced miR-455-3p expression and significantly upregulated lncRNA HOTTIP and CCL3 expression in OA cartilage tissues and chondrocytes. The expression of HOTTIP and CCL3 was increased in chondrocytes treated with interleukin-1β (IL-1β) in vitro. Knockdown of HOTTIP promoted cartilage-specific gene expression and suppressed CCL3. Conversely, HOTTIP overexpression reduced cartilage-specific genes and increased CCL3. Notably, HOTTIP negatively regulated miR-455-3p and increased CCL3 levels in human primary chondrocytes. Mechanistic investigations indicated that HOTTIP functioned as ceRNA for miR-455-3p enhanced CCL3 expression. Taken together, the ceRNA regulatory network of HOTTIP/miR-455-3p/CCL3 plays a critical role in OA pathogenesis and suggests HOTTIP is a potential target in OA therapy.

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microRNA-Seq of Gallus gallus embryo tissues: Undetermined Presomitic Mesoderm (PSM), Determined PSM, and Limb bud

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Project description:MicroRNA-455 is a key microRNA in cartilage, strongly induced by Sox9. In order to understand its function, we have overexpressed (miR-455-3p mimic) or inhibited (miR-455-3p inhibitor) miR-455 compared to appropriate controls and measured gene expression.

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