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RNA-seq analysis of differentially expressed genes between stromal vascular fraction (SVF) from inguinal white adipose tissue (iWAT) of mice treated with medicinal herb extract (MHE) and Phosphate-buffered saline (PBS)

ABSTRACT: RNA-seq analysis of differentially expressed genes between stromal vascular fraction (SVF) from inguinal white adipose tissue (iWAT) of mice treated with medicinal herb extract (MHE) and Phosphate-buffered saline (PBS)

PROVIDER: PRJNA1170938 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30937754_1.fastq.gz	Fastqsanger.gz
	SRR30937754_2.fastq.gz	Fastqsanger.gz
	SRR30937755_1.fastq.gz	Fastqsanger.gz
	SRR30937755_2.fastq.gz	Fastqsanger.gz
	SRR30937756_1.fastq.gz	Fastqsanger.gz

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RNA-seq analysis of differentially expressed genes between stromal vascular fraction (SVF) from inguinal white adipose tissue (iWAT) of mice treated with medicinal herb extract (MHE) and Phosphate-buffered saline (PBS)

Project description:To assess the influences of a crude medicinal herb extract (MHE) on the immune composition and function in inguinal white adipose tissue (iWAT), we isolated iWAT stromal vascular fractions (SVFs) from control (PBS) and MHE-treated mice and performed RNA-seq analysis. iWAT SVF of mice treated with MHE was defined as the treatment group. Phosphate-buffered saline (PBS) treatment was used as the control group. Then RNA-Seq experiment was performed by OE Biotech Co., Ltd. (Shanghai, China) to analyze gene expression changes in iWAT SVF.

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Project description:We previously derived a doxycycline-inducible (Tet-On) lineage-tracing model that allows for the indelible labeling of Pdgfrb-expressing perivascular cells in adipose tissue of adult mice (PdgfrbrtTA; TRE-Cre; Rosa26RmT/mG; herein, MuralChaser mice). Prior to exposing animals to doxycyline, all cells within the stromal-vascular fraction (SVF) of adult inguinal WAT (iWAT) express membrane tdTomato from the Rosa26 locus. Following 9 days of exposure to doxycycline-containing chow diet, Cre-mediated excision of the loxP-flanked tdTomato cassette occurs in Pdgfrb-expressing cells, and membrane-bound GFP (mGFP) expression is constitutively activated. We set out to test the hypothesis that Pdgfrb-expressing perivascular cells in inguinal WAT of adult mice are heterogeneous, with subpopulations harboring functionally distinct phenotypes. To this end, we performed single cell RNA-sequencing of mGFP+ cells isolated from iWAT of lean (chow fed) 8 weeks-old male MuralChaser mice following 9 days of doxycycline exposure.

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