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Expression profiling of purified cells after (mature) paneth cell depletion.

ABSTRACT: Expression profiling of purified cells after (mature) paneth cell depletion.

PROVIDER: PRJNA1185731 | ENA |

REPOSITORIES: ENA

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Expression profiling of purified cells after (mature) paneth cell depletion.

Project description:We used transgenic mice expressing diphtheria toxin in a paneth cell specific manner to eablate paneth cells, but found that we could still detect and also purify (paneth) cells in the transgenic mice. We purified this population and a control WT population of paneth cells for expression profiling, with the goal to check if the diphtheria toxin expression system had low penetrance or if our PC population in TG mice was immature , and thus did not yet express the active diphtheria toxin.

2025-02-26 | GSE281800 | GEO

Gene expression by RNA-seq in purified paneth cells in antibiotics microbiome depleded mice

Project description:The study of how gene expression in paneth cells changes after TNF admistration in mice were the microbiome has been depleted with broad spectrum antibiotics. The depletion of the microbiome excludes the occurence of indirect effects mediated through it by TNF on PC gene expression, only direct TNF effecs on crypt cells are measured

2023-12-22 | GSE237588 | GEO

Paneth cell gene expression profiles after zinc suplementation - exp1

Project description:Mice were given zinc as a salt (ZnSO4; 25 mM) in the drinking water for 7 days. Expression profiling with RNA-seq was performed on purified paneth cell to investigate the genome wide effects of zinc supplemenation on the paneth cell gene expression profile

2025-02-26 | GSE281798 | GEO

Gene expression by RNA-seq in purified paneth cells in antibiotics microbiome depleded mice

Project description:Gene expression by RNA-seq in purified paneth cells in antibiotics microbiome depleded mice

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Paneth cell gene expression profiles after zinc suplementation in TNF context

Project description:Mice were given zinc as a salt (ZnSO4; 25 mM) in the drinking water for 7 days, then mice were injected with TNF and sampling was done 3 hours after injection. Expression profiling with RNA-seq was performed on purified paneth cellq to investigate the genome wide effects of zinc supplemenation on the paneth cell gene expression profile in the conext of TNF

2025-02-26 | GSE281799 | GEO

Mouse duodenal and ileal Paneth cell single cell RNA sequencing

Project description:An indepth analysis of Paneth cell transcriptome at single cell level has not been available. Existing intestinal epithelial cell scRNA dataset contain many cell types, where Paneth cells represent a small portion. We used a flow cytometry based approach to enrich and isolate relatively pure Panethc cells from a newly developed Paneth cell reporter mouse line (Lyz1-3'UTR-IRES-CreER; Rosa26R-tdTomato). Single cell RNA sequencing was performed on purified duodenal and ileal Paneth cells of mice housed under specific pathogen free condition.

2023-09-01 | GSE237327 | GEO

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Project description:Paneth cells

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Proteomic profiling of mouse Paneth cells microdissected from small intestinal tissue

Project description:Paneth cells are antimicrobial peptide-secreting cells located at the base of the crypts of the small intestine. The proteome of Paneth cells is not well defined because of their co-existence with stem cells making it difficult to culture Panth cells alone in vitro. Using a simplied toluidine blue O method for staining mouse intestinal tissue, laser capture microdissection (LCM) to isolate cells from the crypt region and surfactant assisted one pot protein digestion, we identified more than 1,300 proteins from crypts equivalent to 18,000 cells. Compared with the proteomes of villi and smooth muscle regions, the crypt proteome is highly enriched in defensins, lysozymes and other antimicrobial peptides that are characteristic of Paneth cells. The sensitivity of the LCM-based proteomics approach was also assessed using a smaller number of cell equivalent tissues, a comparable proteomic coverage can be achieved with 3,600 cells. This work is the first proteomics study of intestinal tissue enriched with Paneth cells. The simplied workflow enables profiling of Paneth cell associated pathological changes at the proteome level directly from frozen intestinal tissue. It may also be useful for proteomics studies of other spatially resolved cell types from other tissues.

2022-10-12 | PXD034143 | Pride

Gene expression by RNA-seq in purified paneth cells of WT and panetch cell specific P55 KO mice 3 hours after TNF

Project description:The study of how gene expression in paneth cells changes 3 hours after TNF admistration in mice were the TNFR1 (P55) genes was deleted in paneth cells using Defa6-cre.

2023-12-15 | GSE237759 | GEO

The effect of Dinc defficiency on the mouse paneth cell transcriptome

Project description:We invstigated the effect of zinc depletion on the mouse intestine, and specifically on paneth cells. Mice ere placed on a zinc depleted diet for 7 weeks after which the the effects of dietary zinc shortage on paneth cells were investigated. A zinc is an essential element for correct paneth cell function and zinc defficiency is a human and animal health problem investigating the PC transcriptom my provide valuable insights into the exact mechanism.

2025-02-26 | GSE281801 | GEO

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