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Expression changes in the XY fetal gonads due to iron deficiency

ABSTRACT: Expression changes in the XY fetal gonads due to iron deficiency

PROVIDER: PRJNA1212543 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32044500_1.fastq.gz	Fastqsanger.gz
	SRR32044500_2.fastq.gz	Fastqsanger.gz
	SRR32044501_1.fastq.gz	Fastqsanger.gz
	SRR32044501_2.fastq.gz	Fastqsanger.gz
	SRR32044502_1.fastq.gz	Fastqsanger.gz

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Expression changes in the XY fetal gonads due to iron deficiency

Project description:Iron plays an important role in epigenome regulation by acting as a cofactor.We developed a method to enrich pre-Sertoli (Nr5a1 positive(+)) cells from mouse embryonic gonads carrying Nr5a1-hCD271 transgene. To examine mRNA expression in pre-Sertoli cells treated with the iron chelator Deferoxamine (Dfo), pre-Sertoli cells were purified and introduced into RNA seq analyses. As a result, we found that the expression of many genes, including Sry, changes due to iron deficiency.

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Expression analysis of Wv/Wv fetal gonads

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Project description:Following sex determination, XY and XX gonads develop into a testis and an ovary, respectively. Depending on the sex of the gonad, resident germ cells will subsequently be committed to either spermatogenesis or oogenesis. In this study we took advantage of the Wv/Wv mouse genetic model, in which gonads are almost devoided of germ cells, to uncover gene expression underlying fetal germ cell development. Male and Female gonads were collected at 12, 14 and 16 days post coitum (dpc) from wild-type and Wv/Wv mouse embryos. Total RNAs were extracted and hybridized on Affymetrix microarrays. Expression signals from male and female gonads or from wild-type and mutant gonads were compared to identify sexually dimorphic genes as well as genes expressed in germ cell during fetal gonad development.

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Transcription profiling of XX and XY sorted mouse somatic support cells and XX and XY mouse whole gonads to identify potential new mammalian gonadal sex determining genes.

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