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Transcriptional changes of Tex10 in purified primordial gem cell-like cells (PGCLCs) at day2 and day6

ABSTRACT: Transcriptional changes of Tex10 in purified primordial gem cell-like cells (PGCLCs) at day2 and day6

PROVIDER: PRJNA1216290 | ENA |

REPOSITORIES: ENA

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Transcriptional changes of Tex10 in purified primordial gem cell-like cells (PGCLCs) at day2 and day6

Project description:Testis-specific transcript 10 (Tex10) is highly expressed in the testis, embryonic stem cells (ESCs), and primordial germ cells (PGCs). We previously generated a Tex10 knockout mouse model demonstrating its critical roles in ESC pluripotency and preimplantation development. Here, using conditional knockout mice and dTAG-degron ESCs, we show Tex10 is required for spermatogenesis and ESC-to-PGCLC differentiation. Specifically, Tex10-null spermatocytes arrest at metaphase I, compromising round spermatid formation. Tex10 depletion and overexpression compromise and enhance ESC-to-PGCLC differentiation, respectively. Mechanistically, bulk and single-cell RNA sequencing reveals that Tex10 depletion downregulates genes involved in pluripotency, PGC development, and spermatogenesis while upregulating genes promoting somatic programs. Chromatin occupancy study reveals that Tex10 binds to H3K4me3-marked promoters of Psmd3 and Psmd7, negative regulators of Wnt signaling, and activates their expression, thereby restraining Wnt signaling. Our study identifies Tex10 as a previously unappreciated factor in spermatogenesis and PGC development, offering potential therapeutic insights for treating male infertility.

2025-02-01 | GSE288151 | GEO

Genome-wide maps of Tex10 and chromatin state in Day 2 primordial germ cell-like cells (PGCLCs)

Project description:We report the Tex10 binding and genome-wide map of H3K4me3 modification in Day 2 PGCLCs by chromatin immunoprecipitation sequencing.

2023-06-14 | GSE180799 | GEO

Tex10 in day2 embryonic bodies (EBs)

Project description:Tex10 in day2 embryonic bodies (EBs)

| PRJNA1216287 | ENA

Tex10 in day2 embryonic bodies (EBs)

2025-02-01 | GSE288153 | GEO

Tex10 in primordial gem cell-like cell (PGCLC) specification and spermatogenesis

Project description:Tex10 depletion compromises the PGCLC specification and spermatogenesis

2023-06-14 | GSE180800 | GEO

Tex10 in primordial gem cell-like cell (PGCLC) specification and spermatogenesis

Project description:Tex10 in primordial gem cell-like cell (PGCLC) specification and spermatogenesis

| PRJNA749589 | ENA

Genome-wide maps of Tex10 and chromatin state in Day 2 primordial germ cell-like cells (PGCLCs)

Project description:Genome-wide maps of Tex10 and chromatin state in Day 2 primordial germ cell-like cells (PGCLCs)

| PRJNA749588 | ENA

Day2

Project description:Day2 test

| PRJEB47166 | ENA

Tex10 in spermatogenesis

Project description:Conditional Tex10 knockout compromises the spermatogenesis

2023-06-14 | GSE190072 | GEO

Gastruloid-derived Primordial Germ Cell-like Cells (Gld-PGCLCs) develop dynamically within integrated tissues

Project description:Primordial Germ Cells (PGCs) are a rare population of cells specified early in embryonic development that give rise to gametes - sperm and egg cells. Current in vitro systems to derive PGC-like cells (PGCLCs) from pluripotent cells use large embryoid bodies, exposed to signalling cocktails, that do not recapitulate the native embryonic environment during PGC formation. Here we show that mouse gastruloids, three-dimensional in vitro models of gastrulation, generate a population of Gastruloid-derived PGC-like cells (Gld-PGCLCs) that resemble early PGCs in vivo. Importantly, the conserved organisation of mouse gastruloids leads to coordinated spatial and temporal localisation of Gld-PGCLCs relative to surrounding somatic cells, even in the absence of exogenous PGC-specific signalling or extraembryonic tissues. In gastruloids, self-organised interactions between cells and tissues, including the endodermal epithelium, enables the specification, migration, and subsequent maturation of a pool of Gld-PGCLCs. As such, mouse gastruloids represent a new source of PGCLCs in vitro and, due to their inherent co-development, serve as a novel model of dynamic PGC development within integrated tissue environments.

2023-08-07 | GSE228406 | GEO

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