Project description:This study aimed to determine the metabolic response of growing Fleckvieh bulls fed diets low in crude protein (CP) supplemented with rumen-protected methionine (RPM). In total, 69 bulls were assigned to three dietary treatments (n=23/group): Positive control (CON; 13.7% CP; 2.11 g methionine/kg DM) and negative control deficient in crude protein (RED; 9.04% CP; 1.56 g methionine/kg DM) with supplemented RPM (RED+MET; 9.04% CP; 2.54 g methionine/kg DM). At slaughter, samples of liver, muscle and blood serum were taken and underwent subsequent metabolomics profiling using a UHPLC-QTOF-MS system. A total of 6,540 features could be detected. 70 metabolites in the liver, six metabolites in muscle and 60 metabolites in blood serum were affected (p < 0.05) due to die-tary treatments. In total, five metabolites could be reliably annotated and were thus subject to subsequent univariate analysis. Reduction in dietary CP had hardly any effect on metabolite abundance in target tissues of both RED and RED+MET bulls as compared CON bulls. Addition of RPM altered hepatic anti-oxidant status in RED+MET bulls as compared to both RED and CON bulls (cysteine glutathione disulfide) . Results exemplify nutrient partitioning in growing Fleckvieh bulls: bulls set maintenance as prevailing metabolic priority (homeostasis) and as a second priority, nutrient trafficking is directed towards special metabolic functions, such as an-ti-oxidant pathways.

2023-07-07 | MSV000092367 | MassIVE

RNA-seq analysis of differential gene expression in rumen papillae from Holstein Friesian bulls undergoing diet restriction and compensatory growth

Project description:The objective of this study was to examine the effect of dietary restriction and subsequent re-alimentation induced compensatory growth on the global gene expression profile of ruminal epithelial papillae. Holstein Friesian bulls (n=38) were assigned to one of two groups: restricted feed allowance (RES; n=19) for 125 days (Period 1) followed by ad libitum access to feed for 55 days (Period 2) or (ii) ad libitum access to feed throughout (ADLIB; n=19). All bulls received the same diet of 70% concentrate 30% grass silage through out the experimental trial,with the amount of feed provided different dependent on each treatment group. At the end of Period 1, 9 animals from each treatment group were slaughtered, with 10 animals from each treatment slaughtered at the end of Period 2. Rumen epithelium was collected from all animals within thirty minutes of slaughter. RNA was extracted and rumen epithelium gene expression was examined using RNAseq technology on all samles collected (end of Period 1: 9 samples each from ADLIB and RES groups; end of Period 2: 10 samples each from ADLIB and RES groups). Genes identified as differentially expressed in response to both dietary restriction and subsequent compensatory growth included those involved in processes such as cellular interactions and transport, protein folding and gene expression, as well as immune response. This information can be exploited in genomic breeding programmes to assist selection of cattle with a greater ability to compensate following a period dietary restriction.

2017-06-09 | GSE89162 | GEO

Jersey and Holstein cow rumen contents

Project description:Jersey and Holstein cow rumen contents Metagenome

| PRJNA869873 | ENA