Project description:The study was designed to test the global impact of negative supercoiling on gene expression in model archaeon T. kodakarensis. Exogenous wild type GyrA or GyrAY119F and wild type GyrB from Thermotoga maritima was expressed under the constitutive PhmtB promoter in T. kodakarensis cells.
Project description:Interventions: Gold Standard:the pathological biopsy results after resection of colorectal mass;Index test:elasticity score; strain ratio
Primary outcome(s): elasticity score;strain ratio;the pathological biopsy results after resection of colorectal mass
Study Design: Diagnostic test for accuracy
Project description:The apratoxin gene cluster was recovered from fosmid DNA library. The gene set responsible for the biosynthesis of the polyketide backbone was introduced in E. coli BAP strain and expressed at 30C for 24 hours. Two sets of control and experimental samples with culture broth pH adjusted at 8 and 12 respectively was extracted with ethyl acetate and dried.