Project description:Whole blood and spleen tissue was collected from naïve untreated mice. We compared the gene expression profiles of naïve autoimmunity prone BQ.Ncf1.m1J mutated mice and wild type BQ mice. The samples from specific pathogen-free animal facility were collected in two sample sets (marked I and II respectively) and the germ-free facility samples (marked GF / germ free) formed the third analyzed batch. The data revealed a pronounced type I interferon signature in the Ncf1.m1J mutated mice housed in both conditions.
Project description:Whole blood and spleen tissue was collected from naïve untreated mice. We compared the gene expression profiles of naïve autoimmunity prone BQ.Ncf1.m1J mutated mice and wild type BQ mice. The samples from specific pathogen-free animal facility were collected in two sample sets (marked I and II respectively) and the germ-free facility samples (marked GF / germ free) formed the third analyzed batch. The data revealed a pronounced type I interferon signature in the Ncf1.m1J mutated mice housed in both conditions. RNA was isolated and globin removal was performed for all samples. The cells were not stimulated or selected ex vivo.
Project description:N6 methyladenoside (m6A) sequencing was performed on induced Treg cells, Tnaive cells, CD4+ T cells from germ-free mice and CD4+ T cells from specific pathogen free mice. We found that there are 465 genes with increased m6A levels and 4083 genes with decreased m6A levels in iTregs compared to Tnaïve cells. We found that there are 291 genes with increased m6A levels and 344 genes with decreased m6A levels in germ-free CD4+ T-cells compared to T cells from specific pathogen free mice
Project description:mRNA expression from wild type and gp130F/F mice antrum at 4 weeks of age in specific pathogen free (SPF) and germ free (GF) conditions
