Project description:Genome-wide methylation profiling of mouse 3T3-L1 Cells comparing control wildtypes with cells stable transfected with slincRAD shRNA8 (sh8). Two-condition experiment, 3T3-L1 wildtype vs. slincRAD-shRNA8 stable transfected cells before and after Adipogenesis. One replicate per array.

2016-07-01 | E-GEOD-81739 | biostudies-arrayexpress

Genome-wide profiling of Prmt5 binding during 3T3-L1 adipogenesis

Project description:This study aims to determine the unique role of Prmt5 during adipogenesis by profiling Prmt5’s chromatin binding sites genome-wide at three time points during 3T3-L1 differentiation.

2023-09-08 | GSE148117 | GEO

Detection of microRNA expression during 3T3-L1 pre-adipocyte differentiation using a custom made microarray chip.

Project description:Differentiation of 3T3-L1 cells into adipocytes involves a highly orchestrated series of events including clonal expansion, growth arrest and terminal differentiation. The mechanisms coordinating these different steps are not yet fully understood. Here we investigated whether micro (mi)RNAs play a role in this process. Microarray analysis was performed to detect miRNA expression during 3T3-L1 preadipocyte differentiation. Several miRNAs, including let-7, were up-regulated during 3T3-L1 adipogenesis. Ectopic introduction of let-7 into 3T3-L1 cells inhibited clonal expansion as well as terminal differentiation. The mRNA encoding high mobility group AT-hook 2 (HMGA2), a transcription factor that regulates growth and proliferation in other contexts, was inversely correlated with let-7 levels during 3T3-L1 cell adipogenesis, and let-7 markedly reduced HMGA2 concentrations. Knockdown of HMGA2 inhibited 3T3-L1 differentiation. These results suggest that let-7 plays an important role in adipocyte differentiation and that it does so in part by targeting HMGA2, thereby regulating the transition from clonal expansion to terminal differentiation.

2009-05-26 | GSE16229 | GEO

Mus musculus

Project description:3T3-L1-NC V.S 3T3-L1-LIGHT RNA-seq

| PRJNA451383 | ENA

Effect of Paral1 deficiency on gene expression in 3T3-L1 cells

Project description:Paral1 is a novel adipocyte-specific lincRNA upregulated during adipogenesis of 3T3-L1 cells. Knockdown of Paral1 by siRNA transfection in 3T3-L1 cells followed by transcriptomic analyses was performed.

2017-11-15 | GSE97241 | GEO

Expression data from Fbxl10 overexpressing 3T3-L1 cells

Project description:Target genes of Fbxl10 during 3T3-L1 adipogenesis was analyzed 3T3-L1 cells overexpressing Fbxl10 using retrovirus system containing LTR promoter were differentiated and RNA was extracted at day 2 of differentiation

2015-03-04 | E-GEOD-64154 | biostudies-arrayexpress

Expression data from Fbxl10 overexpressing 3T3-L1 cells

Project description:Target genes of Fbxl10 during 3T3-L1 adipogenesis was analyzed

2015-03-04 | GSE64154 | GEO