Project description:microarray experiment to test the gene expression in long term lines of mutator and non-mutator yeast. Here we use an experimental evolution approach to investigate the conditions required for evolution of a reduction in mutation rate and the mechanisms by which populations tolerate the accumulation of deleterious mutations. We find that after ~6700 generations four out of eight experimental mutator lines had evolved a decreased mutation rate.
Project description:microarray experiment to test the gene expression in long term lines of mutator and non-mutator yeast. Here we use an experimental evolution approach to investigate the conditions required for evolution of a reduction in mutation rate and the mechanisms by which populations tolerate the accumulation of deleterious mutations. We find that after ~6700 generations four out of eight experimental mutator lines had evolved a decreased mutation rate. 2 condition experiment, derived experimental evolution strains compared to their ancestor strain. We compared the expression profile of one of the mutator lines (m8) after 6700 generations with its mutator ancestor, and as a control, an evolved non mutator after 6700 generations was compared to to its non-mutator ancestor. In order to prepare cells for expression microarray, glass tubes containing 3 ml of YPD were inoculated from overnight cultures, and grown until the OD600 was approximately 0.3.
Project description:In this study, we performed RNAseq of PolyA enriched RNA from budding yeast cells that were either wild-type or having the mcd1-1 mutation. By obtaining over 200 million reads of sequences from PolyA RNA, we mapped greater than 30 million reads per sample to the yeast genome (S288C R64-2-1_20150113)and generated transcriptomic profiles of wild-type and mcd1-1 mutant cells. Comparison of the wild type and mutant transcriptomes revealed that 11.44% genes were upregulated, 4.99% were downregulated and 83.57% were unaffected in the mutant with a fold change ≥2.0 and Padj value <0.05. When the alterations in gene expression were analyzed with respect to chromosomal position, preferential derepression of subtelomeric (located within 20 kilo bases from the chromosome end) genes was observed in the mutant.
