Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Saccharomyces cerevisiae W303

ABSTRACT: Mutation accumulation in mismatch repair deficient yeast

PROVIDER: PRJNA209500 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR922108.fastq.gz	Fastqsanger.gz
	SRR922109.fastq.gz	Fastqsanger.gz
	SRR922118.fastq.gz	Fastqsanger.gz
	SRR922119.fastq.gz	Fastqsanger.gz
	SRR922353.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 23

Similar Datasets

Saccharomyces cerevisiae

Project description:Mutation accumulation and the yeast transcriptome

| PRJNA99365 | ENA

Saccharomyces cerevisiae

Project description:Mismatch oligonucleotides in yeast

| PRJNA114117 | ENA

Saccharomyces cerevisiae cells: W303-1A vs W303-1A delta MSN2, MSN4

Project description:Transcriptional profiling of Saccharomyces cerevisiae cells comparing the W303-1A wildtype with the W303-1A double mutant for MSN2 and MSN4 during zinc deficient conditions Keywords: Genetic modification with zinc limitation

2008-12-23 | GSE11878 | GEO

Saccharomyces cerevisiae W303

Project description:Mechanism of homology search expansion during recombinational DNA break repair

| PRJNA1046707 | ENA

Saccharomyces cerevisiae

Project description:Heterochronic Evolution Reveals Modular Timing Changes in Budding Yeast Transcriptomes (Mutation Accumulation Lines)

| PRJNA133379 | ENA

Expression data from Saccharomyces cerevisiae strains carrying the rna14-1 or the rna15-1 allele

Project description:In the yeast Saccharomyces cerevisiae, cleavage factor I (CFI) and cleavage and polyadenylation factor (CPF) build the core of the transcription termination machinery. CFI comprises the Rna14, Rna15, Pcf11, and Clp1 proteins, as well as the associated Hrp5 RNA-binding protein. We found that CFI participates in the DNA damage response and that rna14-1 shows synthetic growth defects with mutants of different repair pathways, including homologous recombination, non-homologous end joining, post replicative repair, mismatch repair, and nucleotide excision repair, implicating that impaired RNAPII termination and 3â-end processing decreases the cellular tolerance for DNA damage. Beyond replication progression defects, we found that bypass of the G1/S checkpoint in rna14-1 cells leads to synthetic sickness, accumulation of phosphorylated H2A, as well as increase in Rad52-foci and in recombination. Our data provide evidence that CFI dysfunction impairs RNAPII turnover, leading to replication hindrance and lower tolerance to exogenous DNA damage. These findings underscore the importance of coordination between transcription termination, DNA repair and replication in the maintenance of genomic stability. S. cerevisiae strains were grown in YPAD liquid culture at 30Â°C, total RNA was isolated and hybridized on Affymetrix microarrays.

2014-03-31 | E-GEOD-50947 | biostudies-arrayexpress

Expression data from Saccharomyces cerevisiae strains carrying the rna14-1 or the rna15-1 allele

Project description:In the yeast Saccharomyces cerevisiae, cleavage factor I (CFI) and cleavage and polyadenylation factor (CPF) build the core of the transcription termination machinery. CFI comprises the Rna14, Rna15, Pcf11, and Clp1 proteins, as well as the associated Hrp5 RNA-binding protein. We found that CFI participates in the DNA damage response and that rna14-1 shows synthetic growth defects with mutants of different repair pathways, including homologous recombination, non-homologous end joining, post replicative repair, mismatch repair, and nucleotide excision repair, implicating that impaired RNAPII termination and 3’-end processing decreases the cellular tolerance for DNA damage. Beyond replication progression defects, we found that bypass of the G1/S checkpoint in rna14-1 cells leads to synthetic sickness, accumulation of phosphorylated H2A, as well as increase in Rad52-foci and in recombination. Our data provide evidence that CFI dysfunction impairs RNAPII turnover, leading to replication hindrance and lower tolerance to exogenous DNA damage. These findings underscore the importance of coordination between transcription termination, DNA repair and replication in the maintenance of genomic stability.

2014-03-31 | GSE50947 | GEO

Saccharomyces cerevisiae W303

Project description:Genetic interaction network in continuous culture-grown yeast populations

| PRJNA694549 | ENA

Saccharomyces cerevisiae W303

Project description:Highly-automated, high-throughput replication of yeast-based logic circuit design assessments

| PRJNA784977 | ENA

Saccharomyces cerevisiae W303

Project description:Constitutive turnover of histone H2A.Z at yeast promoters requires the preinitiation complex

| PRJNA271808 | ENA