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Nasonia vitripennis

ABSTRACT: Parasitoid wasps are natural enemies of many arthropods, including houseflies

PROVIDER: PRJNA13647 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	AAZX01.dat.gz	Other
	AAZX01.fasta.gz	Fasta.gz
	AAZX01.master.dat	Other
	WELF01.dat.gz	Other

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Project description:Parasitoid wasps are natural enemies of many arthropods, including houseflies

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Genome-scale methylation changes induced by photoperiods in Nasonia vitripennis

Project description:Seasonal photoperiodic changes have strong impact on development in Nasonia vitripennis. Here, Using high-throughput Reduced Representation Bisulfite Sequencing (RRBS) and single-molecule-based sequencing, we generated DNA methylation maps of female wasps maintained in long vs short day. We have identified differential methylated loci that encode the photoperiodic change. analysis of DNA methylation in female wasps maintained in long vs short day, using RRBS followed by Illumina sequencing

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Project description:The venom gland and ovary transcriptome of the parasitoid wasp Nasonia vitripennis

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Genome-scale methylation changes induced by photoperiods in Nasonia vitripennis

2014-09-01 | GSE44869 | GEO

The transcriptomic basis of oviposition behaviour in the parasitoid wasp Nasonia vitripennis

Project description:For many behaviours studied at the phenotypic level, we have little or no idea of where to start searching for “candidate” genes: the transcriptome provides such a starting point. Here we consider transcriptomic changes associated with oviposition in the parasitoid wasp Nasonia vitripennis. Oviposition is a key behaviour, as females are faced with a variety of decisions that will impact offspring fitness. These include choosing between hosts of differing quality, as well as deciding on clutch size and offspring sex ratio. We compared the whole-body transcriptomes of resting or ovipositing female Nasonia using a “DEEP-Sage” gene expression approach on the Illumina sequencing platform.

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Nasonia vitripennis strain:AsymCX

Project description:Genome sequencing of the parasitoid wasp Nasonia vitripennis

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Profiling of expression level in adult female Nasonia vitripennis

Project description:The expression level in the parasitoid Nasonia vitripennis adult female samples was profiled and compared with the methylation pattern. Methylated and non-methylated genes showed markedly different patterns. The expression level was higher for methylated than non-methylated genes. non-nmethylated genes account for 99% of the genes that were not found to be expressed in the adult female RNA-seq data (FPKM < 0.1). Unlike methylated genes, the expression distribution of the non-methylated genes was bimodal with a lower expressed group and a moderately expressed group of genes, indicating that methylation status is not the only determinant of high expression in adult females. Methylated genes also have lower coefficient of variation (CV) of expression level across five developmental stages, suggesting that they are expressed more evenly across development.

2013-08-27 | GSE43422 | GEO

Whole-body transcriptomics of Nasonia vitripennis females as they respond to sex allocation cues

Project description:Linking the evolution of the phenotype to the underlying genotype is a key aim of evolutionary genetics and is crucial to our understanding of how natural selection shapes a trait. Here we consider the genetic basis of sex allocation behaviour in the parasitoid wasp Nasonia vitripennis using a transcriptomics approach. Females allocate offspring sex in line with Local Mate Competition (LMC) theory. Female-biased sex ratios are produced when one or few females lay eggs on a patch. As the number of females contributing offspring to a patch increases, less female-biased sex ratios are favoured. We contrasted the transcriptomic responses of females as they oviposit under conditions known to influence sex allocation: foundress number (a social cue) and the state of the host (parasitised or not). We found, that when females encounter other females on a patch, or assess host quality with their ovipositors, the resulting changes in sex allocation is not associated with significant changes in whole-body gene expression. We also found that the gene expression changes produced by females, as they facultatively allocate sex in response to a host cue and a social cue, are very closely correlated. We expanded the list of candidate genes associated with oviposition behaviour in Nasonia, some of which may be involved in fundamental processes underlying the ability to facultatively allocate sex, including sperm storage and utilisation.

2015-11-11 | GSE74241 | GEO

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