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Nasonia vitripennis

ABSTRACT: Parasitoid wasps are natural enemies of many arthropods, including houseflies

PROVIDER: PRJNA13647 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	JGIL_10.filt.all.vcf.gz	Vcf
	JGIL_10.filt.all.vcf.gz.tbi	Vcf
	SRR10229739.fastq.gz	Fastqsanger.gz
	SRR10229740.fastq.gz	Fastqsanger.gz

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Genome-scale methylation changes induced by photoperiods in Nasonia vitripennis

Project description:Seasonal photoperiodic changes have strong impact on development in Nasonia vitripennis. Here, Using high-throughput Reduced Representation Bisulfite Sequencing (RRBS) and single-molecule-based sequencing, we generated DNA methylation maps of female wasps maintained in long vs short day. We have identified differential methylated loci that encode the photoperiodic change. analysis of DNA methylation in female wasps maintained in long vs short day, using RRBS followed by Illumina sequencing

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Genome-scale methylation changes induced by photoperiods in Nasonia vitripennis

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The transcriptomic basis of oviposition behaviour in the parasitoid wasp Nasonia vitripennis

Project description:For many behaviours studied at the phenotypic level, we have little or no idea of where to start searching for “candidate” genes: the transcriptome provides such a starting point. Here we consider transcriptomic changes associated with oviposition in the parasitoid wasp Nasonia vitripennis. Oviposition is a key behaviour, as females are faced with a variety of decisions that will impact offspring fitness. These include choosing between hosts of differing quality, as well as deciding on clutch size and offspring sex ratio. We compared the whole-body transcriptomes of resting or ovipositing female Nasonia using a “DEEP-Sage” gene expression approach on the Illumina sequencing platform.

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Nasonia vitripennis strain:AsymCX

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RNA from Developmental analysis of DNA Methylation in the Parasitoid Wasp Nasonia vitripennis

Project description:RNA samples to pair with methylation data from the Developmental DNA Methylation in the Parasitoid Wasp Nasonia vitripennis paper

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Profiling of expression level in adult female Nasonia vitripennis

Project description:The expression level in the parasitoid Nasonia vitripennis adult female samples was profiled and compared with the methylation pattern. Methylated and non-methylated genes showed markedly different patterns. The expression level was higher for methylated than non-methylated genes. non-nmethylated genes account for 99% of the genes that were not found to be expressed in the adult female RNA-seq data (FPKM < 0.1). Unlike methylated genes, the expression distribution of the non-methylated genes was bimodal with a lower expressed group and a moderately expressed group of genes, indicating that methylation status is not the only determinant of high expression in adult females. Methylated genes also have lower coefficient of variation (CV) of expression level across five developmental stages, suggesting that they are expressed more evenly across development.

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