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ABSTRACT: Drosophila dKDM5/LID regulates H3K4me3 dynamics at the transcription start site of actively transcribed developmental genes

PROVIDER: PRJNA137545 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR097978.fastq.gz	Fastqsanger.gz
	SRR097979.fastq.gz	Fastqsanger.gz
	SRR097980.fastq.gz	Fastqsanger.gz
	SRR097981.fastq.gz	Fastqsanger.gz
	SRR097982.fastq.gz	Fastqsanger.gz

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Project description:LID is a histone demethylase acting on H3K4me3, a mark related to transcription and found near the transcription start sites (TSS) of the genes. We analyzed where LID is localized and the effects of LID downregulation in the distribution of H3K4me3. Analysis of LID-binding sites in wild type, and of H3K4me3-binding sites in wild type and LID RNAi wing imaginal discs.

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Project description:H3K4me3 is a histone modification related to gene activation. LID is a demethylase acting on this residue and therefore, it could be important for proper expression of genes in Drosophila developing tissues, such as wing imaginal discs We used microarrays to analyse the changes in gene expression after lid depletion by RNAi, both in a wild type background and in a mutant background Two replicates were obtained on Nov 2010 for wild type white drosophila, GFP RNAi control and LID RNAi (on a LID mutant background).

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Project description:Actively transcribed genes insulate Polycomb domains in Drosophila

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