Project description:Leaves of the potatoes grown for 3 and 8 weeks at NT and HT were harvested for total RNA extraction. Total RNA was extracted from 100 mg of ground leaves using the Trizol reagent (MRC) following the manufacturer’s instructions. Five micro gram of each RNA sample were generated for a strand-specific library as previously described. The library was containing inserts of 150-200 bp. For RNA sequencing, paired-end 150 nt sequencing was performed using a HiSeq 4000 platform (Illumina, USA) at Microgen (Korea).
Project description:orf137 and orf320 are Candidates for Mitochondrial Genes Associated with W/alpha and T/beta-type Cytoplasmic Male Sterility in Potatoes
Project description:orf137 and orf320 are Candidates for Mitochondrial Genes Associated with W/alpha and T/beta-type Cytoplasmic Male Sterility in Potatoes
Project description:orf137 and orf320 are Candidates for Mitochondrial Genes Associated with W/alpha and T/beta-type Cytoplasmic Male Sterility in Potatoes
Project description:orf137 and orf320 are Candidates for Mitochondrial Genes Associated with W/alpha and T/beta-type Cytoplasmic Male Sterility in Potatoes
Project description:St (common potato) is a freezing sensitive species unable to cold acclimate. The close wild relative Sc is freezing tolerant and able to cold acclimate. Here we compare the cold transcriptome of these two species with different levels of freezing tolerance. We also identify the putative CBF regulons by comparing the transcriptomes of wild type plants with that of 35S::AtCBF3 transgenic lines in both species. Plants were grown in 16:8 photoperiod. Eight hours after dawn, plants were either transfered to cold or kept in the warn. Wild type S. tuberosum and S. commersonii were grown at 2oC for 2h, 24h and 7 days. Wild type plants grown under warm temperatures for 2h was used as control for 2h cold samples; wild type warm grown plants for 24h were used as controls for 24h and 7 days cold samples. Under warm conditions, S. commersonii 35S::AtCBF3 lines were compared to S. commersonii wild type plants (same thing was done for S. tuberosum).
