ABSTRACT: Primary Central Nervous System Lymphomas. A validation study of array-based Comparative Genomic Hybridization in Formalin-Fixed Paraffin-Embedded Tumor Specimens
Project description:Primary central nervous system lymphomas (PCNSL) are extranodal non-Hodgkin lymphomas arising within brain, spine cord or eyes and represent approximately 3% of all primary brain tumors in adults. PCNSL are histologically homogeneous- more than 90% are diffuse large B cell lymphomas - but clinically heterogeneous. Molecular basis which could explain PCNSL agressiveness are unknown due to paucity of biological material (i.e. stereotaxic biopsies). In order to identify recurrent genomic abnormalities in PCNSL, SNP arrays and CGH arrays were analyzed for 27 and 6 PCNSL cases, respectively. Fresh frozen samples were generally investigated by SNP array (Illumina 370K or 610K microarrays) and formalin-fixed paraffin-embedded tissues by CGH array (Nimblegen and Agilent microarrays) except for 3 frozen samples analyzed by CGHa.
Project description:This SuperSeries is composed of the following subset Series: GSE23384: Gene profiling using archival formalin-fixed paraffin-embedded breast cancer specimens can generate informative microarray data: A comparison with matched fresh fine needle aspiration biopsy samples (FFPE samples) GSE23385: Gene profiling using archival formalin-fixed paraffin-embedded breast cancer specimens can generate informative microarray data: A comparison with matched fresh fine needle aspiration biopsy samples (FNA samples) Refer to individual Series
Project description:Transcriptional profiles of formalin-fixed-paraffin-embedded melanoma metastases including pre-treatment (PRE) and post-treatment (POST) specimens from 50 patients treated with BRAF inhibitors or with BRAF and MEK inhibitors
Project description:To characterize histology-based transcriptional heterogeneity of human hepatoblastoma, Smart-3SEQ was used to perform RNA sequencing of distinct histologic regions isolated by laser capture microdissection from formalin-fixed paraffin-embedded primary human hepatoblastoma specimens.
Project description:Comparative proteome analysis of BL and DLBCL cell lines, cryopreserved and formalin-fixed paraffin-embedded (FFPE) primary tumor specimens by SWATH-MS.
