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Escherichia coli str. K-12 substr. MG1655

ABSTRACT: ChIP-chip of E. coli K-12 MG1655 with antibody against PurR-8myc under various conditions.

PROVIDER: PRJNA142215 | ENA |

REPOSITORIES: ENA

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Genome-scale reconstruction of the PurR regulon reveals its role in the adenine stimulon of Escherichia coli K-12 MG1655

Project description:This SuperSeries is composed of the following subset Series: GSE26588: Transcriptome analysis of E. coli MG1655 GSE26589: ChIP-chip of E. coli K-12 MG1655 with antibody against PurR-8myc under various conditions. Refer to individual Series

2012-01-12 | E-GEOD-26591 | biostudies-arrayexpress

ChIP-chip of E. coli K-12 MG1655 with antibody against PurR-8myc under various conditions.

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Project description:ChIP-chip of E. coli K-12 MG1655 with antibody against ArgR-8myc, Lrp-8myc, and TrpR-8myc under various conditions.

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ChIP-chip of E. coli K-12 MG1655 with antibody against PurR-8myc under various conditions.

Project description:We integrated transcription factor binding regions and mRNA transcript abundance to elucidate the PurR regulon experimentally. To measure transcription factor binding at a genome scale, we employed a ChIP-chip method to derivative strains of E. coli K-12 MG1655 harboring PurR-8myc under various conditions. A four ChIP-chip study under two separate culture conditions. The high-density oligonucleotide tiling arrays used were consisted of 371,034 oligonucleotide probes spaced 25 bp apart (25-bp overlap between two probes) across the E. coli genome.

2011-06-14 | E-GEOD-26589 | biostudies-arrayexpress

Chip-chip from Escherichia coli MG1655 K-12, WT and ∆fnr strains

Project description:Mapping the occupancy of FNR, HNS, and IHF throughout the genome of Escherchia coli MG1655 K-12 using an affinity purified antibody under anerobic growth conditions. We also mapped the binding of the ß subunit of RNA Polymerase under both aerobic and anaerobic growth conditions. As a control, we also performed ChIP-chip on FNR in a ∆fnr mutant strain of Escherchia coli MG1655 K-12. We also examined FNR immunoprecipitation at various FNR concentrations using IPTG and Ptac::fnr (PK8263). The ∆hns/∆stpA strains were also used. Descirbed in the manuscript Genome-scale Analysis of E. coli FNR Reveals the Complexity of Bacterial Regulon Structure

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Transcriptome analysis of E. coli MG1655

Project description:Expression profiling of wild type and purR deletion strains of E. coli K-12 MG1655 under both M9 minimal media and addition of adenine.

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Escherichia coli str. K-12 substr. MG1655

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ArcA Chip-chip from Escherichia coli MG1655 K-12, WT and ∆arcA strains

Project description:Mapping the occupancy of ArcA throughout the genome of Escherchia coli MG1655 K-12 using an affinity purified antibody under anaerobic and aerobic growth conditions. As a control, we also performed ChIP-chip onArcA in a ∆arcA mutant strain of Escherchia coli MG1655 K-12. Described in the manuscript The response regulator ArcA uses a diverse binding site architechture to globally regulate carbon oxidation in E. coli

2013-10-17 | GSE46413 | GEO

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