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Sorghum bicolor

ABSTRACT: RNA-Seq of Sorghum bicolor 9d seedlings in response to osmotic stress and abscisic acid

OTHER RELATED OMICS DATASETS IN: E-GEOD-30249

PROVIDER: PRJNA143783 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR299227.fastq.gz	Fastqsanger.gz
	SRR299228.fastq.gz	Fastqsanger.gz
	SRR299229.fastq.gz	Fastqsanger.gz
	SRR299230.fastq.gz	Fastqsanger.gz
	SRR299231.fastq.gz	Fastqsanger.gz

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E-GEOD-30249

Project description:RNA-Seq of Sorghum bicolor 9d seedlings in response to osmotic stress and abscisic acid

2015-08-26 | E-GEOD-30249 | ExpressionAtlas

RNA-Seq of Sorghum bicolor 9d seedlings in response to osmotic stress and abscisic acid

Project description:This study utilized next generation sequencing technology (RNA-Seq) to examine the transcriptome of sorghum plants challenged with osmotic stress and exogenous abscisic acid (ABA) to elucidate those genes and gene networks that contribute to sorghum's tolerance to water-limiting environments with a long-term aim of developing strategies to improve plant productivity under drought. We examined the mRNA of 9 day old Sorghum bicolor (BTx623) from 2 tissue types (roots and shoots) for 2 treatments (20 uM ABA and 20% PEG) with corresponding controls (0.2M NaOH and H2O) for 27 hrs prior to harvesting, each done in triplicate biological replicates - resulting in 24 unique runs

2011-10-26 | E-GEOD-30249 | biostudies-arrayexpress

RNA-Seq of Sorghum bicolor 9d seedlings in response to osmotic stress and abscisic acid

2011-10-27 | GSE30249 | GEO

Tissue specific expression data in Sorghum bicolor BTx623

Project description:This study used with RNA-Seq to examine the tissue specific expression data within sorghum plants for improving the Sorghum bicolor gene annotation. We examined the RNA from tissues (spikelet, seed and stem) in Sorghum bicolor (BTx623).Total RNAs form each tissues were extracted using SDS/phenol method followed by LiCl purification

2015-03-11 | E-MTAB-4273 | biostudies-arrayexpress

RNA-Seq and BS-Seq of Sorghum bicolor tissues

Project description:This study utilized next generation sequencing technology (RNA-Seq and BS-Seq) to examine the transcriptome and methylome of various tissues within sorghum plants with the ultimate goal of improving the Sorghum bicolor annotation We examined the mRNA of various Sorghum bicolor (BTx623) tissues (flowers, vegitative and floral meristems, embryos, roots and shoots) and bisulfite treated DNA from two root samples

2014-08-29 | E-GEOD-50464 | biostudies-arrayexpress

Sorghum bicolor and Setaria viridis PARE-seq

Project description:Parallel Analysis of RNA Ends (PARE) sequencing reads were generated to validate putative microRNAs and identify cleavage sites in Sorghum bicolor and Setaria viridis.

2020-05-19 | GSE150798 | GEO

RNA-Seq and BS-Seq of Sorghum bicolor tissues

2014-08-29 | GSE50464 | GEO

Genome-wide analysis of abscisic acid biosynthesis, catabolism, and signaling in Sorghum bicolor under saline-alkali stress

Project description:Sorghum (Sorghum bicolor) is the fifth most important cereal crop in the world. It is an annual C4 crop having a high biomass, used widely, and has a strong resistance to stress. Obviously, there are many benefits of planting sorghum on marginal soils such as saline-alkali land.

2020-12-03 | GSE140928 | GEO

Drought-responsive small RNAs in Sorghum bicolor (L.) Moench

Project description:The present study is expected to reveal regulatory network of small RNAs under drought in Sorghum (Sorghum bicolor (L.) Moench). Sorghum genotype drought tolerant (DT) and drought susceptible (DS) were grown at 28-32 degrees C day/night temperature with 12/12 h light/dark period in the phytotron glass house. The fully opened uppermost leaves from control and drought stressed seedlings were sampled and stored at -80 degrees C, and used for generation of a small RNA library. Total RNA was isolated from the leaves using the TRIzol reagent (Invitrogen, USA). Small RNA sequencing libraries were prepared using Illumina Truseq small RNA Library preparation kit following manufacturer's protocol and these libraries were sequenced on GAIIx platform (Illumina Inc., USA). Small RNA reads contaminated with poor-quality and adaptor sequences were trimmed by using the UEA sRNA workbench 2.4- Plant version sequence file pre-processing (http://srna-tools.cmp.uea.ac.uk/). Then, all unique reads were submitted to the UEA sRNA toolkit-Plant version miRCat pipeline (http://srna-tools.cmp.uea.ac.uk/) to predict novel miRNAs from high-throughput small RNA sequencing data.

2014-04-15 | E-MTAB-1630 | biostudies-arrayexpress

RNA-Seq and BS-Seq of Sorghum bicolor tissues

Project description:This experiment contains the subset of data corresponding to sorghum RNA-Seq data from experiment E-GEOD-50464 (http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-50464/), which goal is to examine the transcriptome of various Sorghum bicolor (BTx623) tissues: flowers, vegetative and floral meristems, embryos, roots and shoots. Thus, we expanded the existing transcriptome atlas for sorghum by conducting RNA-Seq analysis on meristematic tissues, florets, and embryos, and these data sets have been used to improve on the existing community structural annotations.

2014-08-29 | E-MTAB-3839 | biostudies-arrayexpress

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