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ABSTRACT: Kc cells from Drosophila melanogaster during ecdysone treatment and CP190 knockdown

PROVIDER: PRJNA144141 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR317176.fastq.gz	Fastqsanger.gz
	SRR317177.fastq.gz	Fastqsanger.gz
	SRR317178.fastq.gz	Fastqsanger.gz
	SRR317179.fastq.gz	Fastqsanger.gz
	SRR317180.fastq.gz	Fastqsanger.gz

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Kc cells from Drosophila melanogaster during ecdysone treatment and CP190 knockdown

Project description:This SuperSeries is composed of the following subset Series: GSE30686: Gene expression analysis of Kc cells from Drosophila melanogaster during ecdysone treatment and CP190 knockdown GSE30740: Distribution of Drosophila insulator proteins after ecdysone treatment in Kc cells Refer to individual Series

2011-10-13 | E-GEOD-30741 | biostudies-arrayexpress

Gene expression analysis of Kc cells from Drosophila melanogaster during ecdysone treatment and CP190 knockdown

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Gene expression analysis of Kc cells from Drosophila melanogaster during ecdysone treatment and CP190 knockdown

Project description:This study examines the changes in genes expression that occur in Drosophila melanogaster during the ecdysone response as well as during RNAi knockdown of the insulator protein, CP190. Analysis was performed in Kc cells after 0, 3, and 48 hours of ecdysone treatment in the presence of either control or CP190 knockdown. Six conditions were analyzed, and each condition was performed for 2 biological replicates making a total of 12 chips. Each chip measures the expression level of 16,637 genes from D.melanogaster with eight, 60-mer probes per gene.

2011-10-13 | E-GEOD-30686 | biostudies-arrayexpress

Distribution of Drosophila insulator proteins after ecdysone treatment in Kc cells

Project description:Here we examine changes in the distribution of Drosophila insulator proteins during the ecdysone response. We performed ChIP-seq analysis in Kc cells at 0, 3, and 48 hours of ecdysone treatment with antibodies against CP190, Su(Hw), dCTCF, and BEAF-32B.

2011-10-14 | GSE30740 | GEO

Distribution of Drosophila insulator proteins after ecdysone treatment in Kc cells

2011-10-13 | E-GEOD-30740 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Gene expression analysis of Kc cells from Drosophila melanogaster during ecdysone treatment and CP190 knockdown

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Project description:Global quantitative proteomics reveals novel factors in the ecdysone signaling pathway in Drosophila melanogaster.

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Gene expression analysis of Drosophila melanogaster S2 cells after RNAi-mediated depletion of the insulator factor CP190

Project description:We analyzed transcriptional effects after depletion of Drosophila melanogaster S2 cells from CP190 via specific dsRNA treatment in comparison to cells treated with control dsRNA.

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Project description:Distribution of Drosophila insulator proteins after ecdysone treatment in Kc cells

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Drosophila Ecdysone Receptor Study

Project description:We used the DamID method to systematically identify the binding sites of Ecdysone Receptor and its heterodimeric partner USP across the whole genome in Drosophila Kc cells. We find that the EcR sites are a subset of the USP sites and that only a proportion are ecdysone regulated from an accompanying ecdysone profiling study. The role of EcR/USP in the ecdysone network appears to be coordinated by the recruitment of many transcription factors as well as signaling molecules. Keywords: DamID, chromatin profiling, DNA microarray

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