Project description:Development of gene expression profile for a seasonal variation study in a natural population of Riba-Roja, in the Ebro River, Spain
Project description:To analyze the different pattern of expression among tissues in D. polymorpha it was used acustom microarray designed in our laboratory using 4057 publicly availabe DNA sequences from Dreissena and other realted genera. Transcriptome profiles were analyszed using gills, digestive gland, gonad and half a body of adult zebra mussel and larvae 24 h pf. The samples analyzed in the microarray were not treated in order to detect the normal pattern of expression in each tissue. A total of 221 transcripts changed significantly their mRNA levels in the different tissues (ANOVA p<0.01, fc ±1.5). The results showed a clear separation on transcriptome profiles between samples with the main changes in gills, larvae and half a body.
Project description:To analyze the different pattern of expression among tissues in D. polymorpha it was used acustom microarray designed in our laboratory using 4057 publicly availabe DNA sequences from Dreissena and other realted genera. Transcriptome profiles were analyszed using gills, digestive gland, gonad and half a body of adult zebra mussel and larvae 24 h pf. The samples analyzed in the microarray were not treated in order to detect the normal pattern of expression in each tissue. A total of 221 transcripts changed significantly their mRNA levels in the different tissues (ANOVA p<0.01, fc ±1.5). The results showed a clear separation on transcriptome profiles between samples with the main changes in gills, larvae and half a body. Non treated animals were use to study different patterns of gene expression in different tissues using gills, digestive gland, gonad from both sexes, and half a body as well as larvae. It was used 2 replicate for each tissue.
Project description:Factorial Microarray Analysis of Zebra Mussel (Dreissena polymorpha) Adhesion Process under the Impact of Multiple Environmental Factors The expression profiles of the zebra mussel byssus unique genes in our cDNA microarray can be influenced by multiple factors. Three environmental factors plus adhesion status were considered as four main factors in this study.
Project description:Mediterranean mussels are a worldwide spread bivalve species with extraordinary biological success. One of the reasons of this success could be the reproduction strategy of bivalves, characterized by the presence of trochophore larvae. Larval development in bivalves has been a topic of raising interest in the scientific community but it deserves much more attention. The principal objective of this work was to study the transcriptomic profile of the ontogeny of M. galloprovincialis analyzing the gene expression in different developmental stages, from oocytes to seed. For this purpose, after conducting a 454 sequencing of transcriptome of mussel hemocytes, adult tissues and larvae, a new DNA microarray comprising sequences of was designed and developed. The studied developmental stages: unfertilized oocytes, veliger (3 days post fertilization; dpf) and pediveliger (20dpf) larvae, settled juveniles (25dpf) and seed (30dpf), showed very different transcriptomic profiles and clustered in groups defining their characteristic gene expression along ontogeny.
