Drosophila melanogaster
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA153093 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Comparison of gene expression in wild type, Oregon[R], Antennapedia[73b], Apterous[56f] Drosophila melanogaster strains. Keywords: other
2003-12-02 | GSE757 | GEO
Project description:Nucleus is a highly structured organelle and contains many functional compartments. While the structural basis for this complex spatial organization of compartments is unknown, a major component of this organization is likely to be the non-chromatin scaffolding called nuclear matrix (NuMat). Experimental evidence over the past decades indicates that most of the nuclear functions are at least transiently associated with the NuMat although the components of NuMat itself are poorly known. Here, we report NuMat proteome analysis from Drosophila melanogaster embryos and discuss its links with nuclear architecture and functions. In the NuMat proteome, we find structural proteins, chaperones related, DNA/RNA binding, chromatin remodeling and transcription factors. This complexity of NuMat proteome is an indicator of its structural and functional significance. Comparison of the 2D profile of NuMat proteome from different developmental stages of Drosophila embryos shows that less than half of the NuMat proteome is constant and rest of the proteins are stage specific dynamic components. This NuMat dynamics suggests a possible functional link between NuMat and the embryonic development. Finally, we also show that a subset of NuMat proteins remain associated with the mitotic chromosomes implicating their role in mitosis and possibly the epigenetic cellular memory. NuMat proteome analysis provides tools and opens up ways to understand nuclear organization and function.
2011-05-05 | PRD000255 | Pride
Project description:We identified genes whose expression is upregulated in adult Drosophila m. brain 48h after a stab lesion by microarray analysis of RNA extracted from injured and intact wild-type brains (Oregon-R).
2022-04-21 | GSE196884 | GEO
Project description:We report the analysis of the transcriptome in Drosophila embryos with two genotypes (1: wild type, 2: embryos from germline clones of a SHMT mutant (allele X238)) and two developmental stages (1: pre-blastoderm, stage 1 and stage 2, 0–1h after egg lay, 2: late blastoderm/cellularisation stage 5, 1.5–2.5 h after egg lay)
2017-08-15 | GSE97557 | GEO
Project description:Transcriptomics analysis of gene expression at three different developmental stages of zebrafish embryos
| PRJNA855894 | ENA
Project description:The aim of this study was to use unbiased transcriptomic analysis to characterize new traits that may explain differences in longevity between short- and long-lived wild-type backgrounds of Drosophila melanogaster – Dahomey and Oregon R, respectively. For the experiment we chose young flies (10 days old) to capture the difference in basal gene expression related to the genotype rather than to age-dependent functional decline. As a source for RNA extraction we used heads and thoraxes (combined) as tissues the most sensitive to aging. The expression of 3939 genes was changed (nearly 26% of the transcriptome, p-value < 0.05), with 1970 being upregulated and 1969 genes being downregulated in the Dahomey background compared to Oregon R. We found that young short-lived Dahomey flies have the traits previously associated with shorten lifespan such as increased lipo-oxidative stress, increased Tor signaling and loss of proteostasis and mitochondrial complex I activity. We hypothesized that all these characteristics are caused by an increase in octopamine signaling that promotes foraging behavior even under laboratory conditions where nutrients are in excess. Our results highlight the importance of controlling the genetic background in aging studies as well as interrogating several different pathways before making conclusions about what causes differences in longevity between different groups or individuals.
2018-06-27 | GSE101465 | GEO
Project description:Gene expression timecourse: SoxN homozygous mutant embryos vs SoxN heterozygous mutant embryos.
| PRJNA205169 | ENA
Project description:We identified genes whose expression is enriched in the D. melanogaster antennal olfactory subsystems that express Odorant receptors (Ors) or Ionotropic receptors (Irs) by microarray analysis of RNA extracted from antennae of wild-type (Oregon R P2) animals, ato mutants (which lack the Ir subsystem) and amos mutants (which lack the Or subsystem).
2021-09-12 | GSE183763 | GEO
Project description:Purpose: The goals of this study are to define transcriptome (RNA-seq) of mouse preimplantation embryos at different stages of development under a range of different environmental conditions. Methods: Mouse preimplantation embro transcriptional profiles were generated using embryos at several different developmental stages using Smart-seq2. Results: RNA-seq analysis finds that there is a highly dynamic pattern of gene expression during the preimplantation period. The sensitiivty to nutrient conditions varies markedly at different stages of development, with 2C embryos more sensitive to pyruvate omission than later stage embryos. Conclusions: Our study represents a comprehensive analysis of the mouse preimplantation development transcriptome, and how pyruvate provision impacts different developmental stages.
2020-10-14 | GSE159484 | GEO
Project description:To investigate the expression profile of three different developental stages of zebrafish embryos, we then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different developmental of zebrafish embryos.
2022-08-17 | GSE207476 | GEO