Project description:This SuperSeries is composed of the following subset Series: GSE33335: Expression data from gastric tissues: Cancer Samples vs. Matched Adjacent Noncancerous Samples GSE33428: Copy number variation profiling of gastric tissues: Cancer Samples vs. Matched Adjacent Noncancerous Samples Refer to individual Series
Project description:Copy number variation profiling of gastric tissues comparing gastric cancer tissues with matched adjacent noncancerous tissues. Goal was to determine the effects of chromosomal imbalances on gene expression and carcinogenesis or progression. 27 pairs of gastric tissues: gastric cancer tissues vs. matched adjacent noncancerous tissues.
Project description:To elucidate gene expression associated with copy number changes, we performed a genome-wide copy number and expression microarray analysis of 25 pairs of gastric tissues. 25 pairs of gastric tissues: gastric cancer tissues vs. matched adjacent noncancerous tissues.
Project description:Copy number variation profiling of gastric tissues comparing gastric cancer tissues with matched adjacent noncancerous tissues. Goal was to determine the effects of chromosomal imbalances on gene expression and carcinogenesis or progression.
Project description:We implemented lncRNA microarray analysis in 5 paired gastric cancer tissues and adjacent noncancerous tissues to identify differential expression of lncRNAs and mRNAs in gastric cancer.
Project description:Total RNA was extracted from human gastric cancer tissues (n=4) and matched adjacent normal tissues (n=4) . RNA samples were analyzed by RNA sequencing based on the manufacturer’s protocols. Briefly, Illumina HiSeq 4000 platform was used to sequence the RNA samples for the subsequent generation of raw data. R package was utilized to select lncRNAs with significantly differential expression based on fold change >2 or <1/2, p value <0.05 between human gastric cancer tissues and matched adjacent normal tissues, and the top 10 upregulated lncRNAs were selected for further study.
