Project description:This SuperSeries is composed of the following subset Series: GSE31895: ChIP with anti-orc2 antibody to identify regions of orc binding in third instar salivary glands of WT and SuUR mutant Drosophila GSE31896: RNAPolII ChIP to find differences between third instar salivary glands of WT and SuUR GSE31897: ChIP with anti-H3K27me3 to compare binding in salivary glands of WT and SuUR Drosophila GSE31898: CGH to ascertain levels of gDNA in third instar salivary glands of various mutant Drosophila GSE31899: ChIP-Seq of ORC2 bound to third instar salivary gland DNA in WT and mutant Drosophila, analyzed by Illumina sequencing GSE33017: Expression profile of third instar larval salivary gland tissue Refer to individual Series
Project description:ChIP was performed to identify regions of gDNA bound by H3K27me3 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that H3K27me3 binds differently in under-replicated in SuUR mutant third instar salivary glands.
Project description:ChIP was performed to identify regions of gDNA bound by H3K27me3 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that H3K27me3 binds differently in under-replicated in SuUR mutant third instar salivary glands. Comparison of H3K27me3 ChIP sample relative to input DNA or control IgG pulldown for two different Drosophila strains
Project description:We analyzed ORC2 ChIP-Seq from hand dissected salivary glands of wandering third instar larvae from OrR or SuUR Drosophila. Goals were to ascertain the difference in binding profile between salivary glands expressing and not expressing the Supressor of UnderReplication protein. One replicate is included for each of OrR (WT) or SuUR salivary glands.
Project description:ChIP was performed to identify regions of gDNA bound by orc2 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that ORC does not localize to regions that are under-replicated in SuUR mutant third instar salivary glands.
Project description:ChIP was performed to identify regions of gDNA bound by RNA polymerase II in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that RNAPolII does not localize to regions that are under-replicated in SuUR mutant third instar salivary glands.
Project description:ChIP was performed to identify regions of gDNA bound by RNA polymerase II in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that RNAPolII does not localize to regions that are under-replicated in SuUR mutant third instar salivary glands. Comparison of RNAPolII ChIP from third instar salivary glands relative to control IgG pulldown for two different Drosophila strains
Project description:ChIP was performed to identify regions of gDNA bound by orc2 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that ORC does not localize to regions that are under-replicated in SuUR mutant third instar salivary glands. Comparison of ORC2 ChIP sample relative to input DNA or control IgG pulldown for two different Drosophila strains
Project description:We analyzed ORC2 ChIP-Seq from hand dissected salivary glands of wandering third instar larvae from OrR or SuUR Drosophila. Goals were to ascertain the difference in binding profile between salivary glands expressing and not expressing the Supressor of UnderReplication protein.
Project description:Comparative genomic hybridization was performed to compare levels of gDNA in third instar salivary glands of Drosophila mutants/nulls in the SuUR and orc proteins, compared with 0-2hr diploid embryo gDNA. This illustrates regions of differential replication in the genome.
