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Brucella suis F5/05-10

ABSTRACT: Brucella suis F5/05-10 Genome sequencing and assembly

PROVIDER: PRJNA173516 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR538751_1.fastq.gz	Fastqsanger.gz
	SRR538751_2.fastq.gz	Fastqsanger.gz
	SRR629757_1.fastq.gz	Fastqsanger.gz
	SRR629757_2.fastq.gz	Fastqsanger.gz

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Integrated proteomic and transcriptomic analyses reveal the roles of Brucella BI-1 in cell division and membrane homeostasis of Brucella suis S2

Project description:To explore the role of Brucella BI-1 in Brucella suis S2, we constructed the Brucella BI-1 deletion mutant strain and its complementary strain. We then determined the effect of Brucella BI-1 deletion on the physiological characteristics of Brucella suis S2 and revealed them via integrated transcriptomic and proteomic analyses. Brucella BI-1 deletion altered the membrane properties of Brucella suis S2 and decreased its resistance to acidic pH, H2O2, polymyxin B, and lincomycin. Additionally, deleting Brucella BI-1 led to defective growth, cell division, and viability in Brucella suis S2. In conclusion, our results revealed that Brucella BI-1 is a bacterial cytoprotective protein involved in membrane homeostasis, cell division, and stress resistance in Brucella suis S2.

2021-02-17 | GSE160911 | GEO

Preliminary characterization of gene expression in European wild boar naturally infected with Brucella suis

2009-09-29 | GSE17492 | GEO

Transcription profiling of European wild boar naturally infected with Brucella suis

Project description:Brucella suis infects macrophages and dendritic cells. Wild boars act as reservoirs and carriers of Brucella suis biovar 2, and there is evidence that wild boar can be the main source of infection for domestic pigs through the venereal route. Transmission through this route could be an important path for disesease dissemination. The result from this study will contribute to the overall understanding of the molecular pathogenic mechanisms involved during Brucella suis infection in European wild boar. Experiment Overall Design: In this study we preliminarily characterized differential gene expression in European wild boar naturally infected with Brucella suis biovar 2 using Microarray hybridization and Real Time RT-PCR analysis. Since Brucella suis acts by infecting macrophages, we used spleen cells to analyze the gene expression response to Brucella suis infection.

2009-10-02 | E-GEOD-17492 | biostudies-arrayexpress

Microarrays of macrophages infected with smooth or rough Brucella suis

2011-06-24 | GSE21117 | GEO

Transcriptome-wide identification of Hfq-associated RNAs in Brucella suis by deep sequencing

Project description:We report the identification of new noncoding RNAs in Brucella suis 1330 and that are associated to the chaperone protein Hfq

2015-11-25 | GSE73621 | GEO

Microarrays of macrophages infected with smooth or rough Brucella suis

Project description:The macrophage-Brucella interaction is critical for the establishment of a chronic Brucella infection. Smooth virulent B. suis strain 1330 (S1330) prevents macrophage cell death. However, rough attenuated B. suis strain VTRS1 induces strong macrophage cell death. To further investigate the mechanism of VTRS1-induced macrophage cell death, microarrays were used to analyze temporal transcriptional responses of murine macrophage-like J774. A1 cells infected with S1330 or VTRS1. Murine J774.A1 macrophages were plated in T75 at 8 x 10^6 cells per flask one day prior to infection, and then infected with B. suis S1330 or VTRS1 at a MOI of 200:1. Total RNAs were isolated by TRIzol and further purified using Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA) at 0, 1, 2, 4, 8, 24, and 48 h post infection. The RNA samples were stored at -80 ºC until an Agilent 2100 BioAnalyzer (Agilent Technologies, Palo Alto, CA) was used to assess the concentrations and quality of RNA samples. Total RNA (20 µg) per sample was used for hybridization with Affymetrix mouse GeneChip 430 2.0 array. Preparation of cDNA, hybridization, quality controls and scanning of the GeneChip 430 2.0 arrays were performed according to the manufacturer's protocol (Affymetrix, Santa Clara, CA) .

2011-06-24 | E-GEOD-21117 | biostudies-arrayexpress

Brucella suis bv. 1 str. 74/10

Project description:Brucella suis bv. 1 str. 74/10 Genome sequencing

| PRJNA47751 | ENA

Brucella suis S2 strain inhibits IRE1/Caspase-12/Caspase-3 pathway-mediated apoptosis of microglia HMC3 by affecting the ubiquitination of CALR

Project description:Neurobrucellosis is a serious complication of brucellosis, which poses a significant threat to human health and quality of life. The origin of this phenomenon is that the patients are more vulnerable to the development of chronic Brucella infection, which is also an important clinical problem. The regulation of Brucella on microglia apoptosis is a primary contributor to chronic neurobrucellosis, nevertheless, the precise molecular mechanism remains incompletely understood. In our research, human microglial clone 3 (HMC3) cells were infected with Brucella suis vaccine strain S2 (B. suis S2) at different MOI for different times to evaluate the influences of B. suis S2 on the IRE1/Caspase-12/Caspase-3 pathway. Subsequently, under the conditions of IRE1/Caspase-12/Caspase-3 pathway was suppressed by B. suis S2, CALR protein was screened by ubiquitin-modified proteomics. Eventually, we infected CALR-overexpression and -knockdown HMC3 cell lines with B. suis S2 to elucidate the molecular mechanism by which the B. suis S2 inhibited HMC3 cells apoptosis. In summary, we confirm that B. suis S2 inhibits apoptosis in HMC3 cells mediated by the IRE1/Caspase-12/Caspase-3 pathway through modulation of CALR ubiquitination. Our study establishes the theoretical foundation for investigating the mechanisms underlying neurobrucellosis and provides a guidance for the clinical treatment of neurobrucellosis.

2025-02-20 | PXD056006 | Pride

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