Project description:We use RNA sequencing technology in a time course study to measure transcript abundance from three developmental stages in cotyledons and five stages in the trifoliate leaf of Glycine max to identify genes with distinct temporal patterns of expression during leaf or cotyledon development. We also examine the diffrences between these two photosynthetic tissues.
Project description:Cytosine methylation is a base modification that is often used by genomes to store information that is stably inherited through mitotic cell divisions. Most cytosine DNA methylation is stable throughout different cell types or by exposure to different environmental conditions in plant genomes. Here, we profile the epigenomes of ~100 Glycine max lines to explore the extent of natural epigenomic variation. We also use these data to determine the extent to which DNA methylation variants are linked to genetic variations.
Project description:We use RNA sequencing technology in a time course study to measure transcript abundance from three developmental stages in cotyledons and five stages in the trifoliate leaf of Glycine max to identify genes with distinct temporal patterns of expression during leaf or cotyledon development. We also examine the differences between these two photosynthetic tissues. Time course Expression analysis of Cotyledon Development and Leaf Development using RNAseq on distinct time points. 3 stages of the cotyledon were sequenced with 3 biological replicates in each stage. Five stages of the leaf, determined by physical appearance and plant developmental stage, were collected from the time of soil emergence to the onset of senescence and were sequenced with 3 biological replicates for each stage.
