Project description:We reported the transcriptional profiles of E.coli expressing antimicrobial peptide LL37 under stress response condition. 4 samples, two groups, one group is under aerobic condition, the other group is under anaerobic condition. One of samples is E.coli which expressed LL37 as induction in each group, another sample is E.coli with no LL37 expression in vivo as control in each group.
Project description:Analysis of undifferentiated keratinocytes or differentiated keratinocytes stimulated with or without human cathelicidin antimicrobial peptide (CAMP) LL37. Results provide insight into the biological effects of CAMP on human keratinocytes. NHEKs were divided into two groups; low calcium (0.05 mM) and high calcium condition (1.6 mM). Then keratinocytes were stimulated with human cathelicidin antimicrobial peptide LL37 at 0, 2.56, and 7.68 M-NM-<M for 12 h to 24 h.
Project description:Analysis of undifferentiated keratinocytes or differentiated keratinocytes stimulated with or without human cathelicidin antimicrobial peptide (CAMP) LL37. Results provide insight into the biological effects of CAMP on human keratinocytes.
2014-08-01 | GSE49472 | GEO
Project description:WGSS of antimicrobial peptide adapted E.coli strains
Project description:To obtain global cellular response of Bacillus subtilis WT W168 against the intrinsically produced antimicrobial peptide YydF, we performed RNA-seq experiments. For this, we synthesized YydF, extrinsically added 0.5µM to logarithmic phase growing cells and harvested cells after 10 min exposure. Experiments were performed in triplicates and non-induced Bacillus subtilis WT W168 was used as reference condition.
Project description:Transcriptional profiling of MatA yeast cells exposed to a cationic antimicrobial peptide (Iztli peptide 1) for 3.5, 7, 15 and 30 min is compared with MatA yeast cells without the peptide Two-condition experiment, MatA cells with peptide (P) and without it (C). Biological samples: 4 control, 4 treatment, independently grown and harvested. Two replicates per array. Please note that [1] there is one raw data file per two replicates, as indicated in the sample description field [2] the Columns 10 and 11 (in the raw data file), labeled as Raw intensity (mean) T1C and Raw intensity (mean) T1P, correspond to the raw intensities for each spot. T1C correspond to control and T1P correspond to treatment with a peptide named Iztli Peptide 1 [3] The replicates can be located by sorting the column standard_name. Each row corresponds to a different gene, two rows for each gene, the first row corresponds to the replicate 1 and the second to the replicate 2.
Project description:Ceragenins are a family of synthetic amphipathic molecules designed to mimic the properties of naturally-occurring cationic antimicrobial peptides (CAMPs). Although ceragenins have potent antimicrobial activity, whether their mode of action is similar to that of CAMPs has remained elusive. Here we report the results of a comparative study of the bacterial responses to two well-studied CAMPs, LL37 and colistin, and two ceragenins with related structures, CSA13 and CSA13.
