Project description:Transcriptional profiling of MIT knockdown plants. MIT is a mitochondrial Fe transporter essential for rice growth and development. The goal was to determine the effects of MIT on global rice gene expression. Control condition experiment, root or shoot of WT vs. MIT knockdown plant. Two replicates each comparison, including a dye swap.
Project description:In order to identify new miRNAs, NAT-siRNAs and possibly abiotic-stress regulated small RNAs in rice, three small RNA libraries were constructed from control rice seedlings and seedlings exposed to drought or salt stress, and then subjected to pyrosequencing.
Project description:In this study, we used a cross-species network approach to uncover nitrogen (N)-regulated network modules conserved across a model and a crop species. By translating gene network knowledge from the data-rich model Arabidopsis (Arabidopsis thaliana, ecotype Columbia-0) to a crop, rice (Oryza sativa spp. japonica (Nipponbare)), we identified evolutionarily conserved N-regulatory modules as targets for translational studies to improve N use efficiency in transgenic plants.
Project description:Transcriptional profiling of MIT knockdown plants. MIT is a mitochondrial Fe transporter essential for rice growth and development. The goal was to determine the effects of MIT on global rice gene expression.
Project description:Artificial miRNA mediated knock-down ago18 transgenic lines [ago18(1), ago18(2)] showed developmentally compromised phenotype in both vegetative and reproductive tissues compared to wild-type control. We have performed small RNA seq to investigate underlying molecular mechanism and to reveal functional role of AGO18 in rice.
Project description:Artificial miRNA mediated knock-down ago18 transgenic lines [ago18(1), ago18(2)] showed developmentally compromised phenotype in both vegetative and reproductive tissues compared to wild-type control. We have performed RNA seq to investigate underlying molecular mechanism and to reveal functional role of AGO18 in rice.
Project description:Transcriptional profiling of young leaves of rice (Oryza sativa) comparing a control transgenic line transformed with p*7GWIWG2(I)-hpt with a double knockdown transgenic line of OsMOM1 genes. Rice has two orthologues (OsMOM1a and OsMOM1b) of Arabidopsis MOM1 gene, which transduces RNA-directed DNA methylation signals to repressive histone modification. To obtain the double knockdown line, at first, single knockdown lines for each OsMOM1 gene were produced with transformation using p*7GWIWG2(I)-hpt-OsMOM1a or p*7GWIWG2(I)-hpt-OsMOM1b. Then a double knockdown plant was selected from progenies of a sexual cross between the single knockdown lines. Both OsMOM1 constructs produce hairpin RNAs and efficiently silence the both target OsMOM1 genes. In the double knockdown line, neither of transcripts from OsMOM1 genes was detected with RNA blot. The double knockdown line might make levels of epigenetic marks (DNA and histone modifications) modified. Goal was to determine the effects of epigenetic change by knockdown of OsMOM1 genes on global gene expression in rice genome.
