Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Time dependent expression profiles of mRNAs and microRNAs in rat milk

ABSTRACT: Time dependent expression profiles of mRNAs and microRNAs in rat milk

PROVIDER: PRJNA188705 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Time dependent expression profiles of mRNAs in rat milk

Project description:We have reported that mRNAs are present in bovine milk. But, it is unknown the roles of milk mRNAs. To clarify the roles of milk mRNAs, experimental animal such as rat study would be needed. However, it is unclear whether rat milk also contains mRNAs. Thus, we have employed microarray to show that rat milk also contains mRNAs.

2014-02-18 | GSE44112 | GEO

Time dependent expression profiles of microRNAs in rat milk

Project description:We have reported that microRNAs are present in human and bovine milk. But, it is unknown the roles of milk miRNAs. To clarify the roles of milk miRNAs, experimental animal such as rat study would be needed. However, it is unclear whether rat milk also contains miRNAs. Thus, we have employed microarray to show that rat milk also contains miRNAs.

2014-02-18 | GSE44113 | GEO

Time dependent expression profiles of mRNAs in rat milk

2014-02-18 | E-GEOD-44112 | biostudies-arrayexpress

Time dependent expression profiles of microRNAs in rat milk

2014-02-18 | E-GEOD-44113 | biostudies-arrayexpress

Time-dependent expression profiles of microRNAs and mRNAs in rat milk whey.

Project description:Functional RNAs, such as microRNA (miRNA) and mRNA, are present in milk, but their roles are unknown. To clarify the roles of milk RNAs, further studies using experimental animals such as rats are needed. However, it is unclear whether rat milk also contains functional RNAs and what their time dependent expression profiles are. Thus, we prepared total RNA from whey isolated from rat milk collected on days 2, 9, and 16 postpartum and analyzed using microarrays and quantitative PCR. The concentration of RNA in colostrum whey (day 2) was markedly higher than that in mature milk whey (days 9 and 16). Microarray analysis detected 161 miRNAs and 10,948 mRNA transcripts. Most of the miRNAs and mRNA transcripts were common to all tested milks. Finally, we selected some immune- and development-related miRNAs and mRNAs, and analysed them by quantitative PCR (in equal sample volumes) to determine their time-dependent changes in expression in detail. Some were significantly more highly expressed in colostrum whey than in mature milk whey, but some were expressed equally. And mRNA expression levels of some cytokines and hormones did not reflect the protein levels. It is still unknown whether RNAs in milk play biological roles in neonates. However, our data will help guide future in vivo studies using experimental animals such as rats.

| S-EPMC3923055 | biostudies-literature

Time dependent expression profiles of mRNAs and microRNAs in rat milk

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-02-18 | GSE44114 | GEO

Time dependent expression profiles of mRNAs and microRNAs in rat milk

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-02-18 | E-GEOD-44114 | biostudies-arrayexpress

Expression profiles of microRNA in bovine raw milk exosome and supernatant without exosome [mRNA array]

Project description:We have reported that microRNAs are present in human, bovine, and rat milk whey. Milk whey miRNAs were resistant to acidic condition and to RNase. Thus, milk miRNAs were thought to be present packaged into membrane vesicles like exosome. However, body fluid miRNAs have been reported that there are in different forms. To clarify which miRNAs species are exist in exosome and which species are exist in another form, we used bovine raw milk and purified total RNA from exosome fraction and ultracentrifugated supernatant fraction, and analyzed by miRNA microarray.

2015-03-09 | GSE61977 | GEO

Expression profiles of microRNA in bovine raw milk exosome and supernatant without exosome [miRNA array]

2015-03-09 | GSE61976 | GEO

Project description:Breast milk is a complex liquid that enriched in immunological components and affect the development of the infant immune system. Exosomes, the membranous vesicles of endocytic origin, are ubiquitously in various body fluids which can mediate intercellular communication. MicroRNAs (miRNAs), a well-defined group of non-coding small RNAs, in human breast milk are packaged inside exosomes. Here, we present the identification of miRNAs in human breast milk exosomes using deep sequencing technology. We found that the immune-related miRNAs are enriched in breast milk exosomes, and are resistant to the general harsh conditions.

2011-12-21 | GSE32253 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data