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Streptococcus pyogenes

ABSTRACT: RNAseq analysis of the serotype M1 group A Streptococcus strain MGAS2221

PROVIDER: PRJNA193454 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR787021.fastq.gz	Fastqsanger.gz
	SRR787022.fastq.gz	Fastqsanger.gz
	SRR787023.fastq.gz	Fastqsanger.gz
	SRR787024.fastq.gz	Fastqsanger.gz

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Subcellular fractionation of Streptococcus pyogenes seotype M1

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Project description:The fasX gene encodes a small regulatory RNA in the group A Streptococcus (GAS). To determine the FasX regulon during GAS exposure to human plasma we compared parental strain MGAS2221 with isogenic fasX mutant strain 2221ΔFasX. Gene expression was analyzed 0, 15, and 60 minutes post-plasma exposure.

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Project description:Two covR mutant derivatives of parental strain MGAS2221 were recovered from mice experimentally infected with MGAS2221 and shown to differ in terms of the number and concentration of secreted proteins. One of the covR mutant strains had a secretion phenotype identical to a covS mutant strain, while the other had a secretion phenotype identical to a constructed covR mutant strain. To further investigate the potential differences between the two covR mutant strains we performed expression microarray analysis. Single cultures of each of the four GAS strains tested were grown in THY broth to early exponential phase (O.D. 0.2). Two volumes of RNA protect were added, the samples incubated at room temperature for 5 minutes, and the bacteria collected through centrifugation. Total RNA was isolated via a mechaniscal disruption method, converted to cDNA, fragmented, labeled, and hybridized to our Affymetrix microarray. Estimates of gene expression were calculated using GCOS software v1.4. Data represent probes for serotype M1.

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