Project description:Genome-wide DNA methylation profiling of U937 and its azacytidine-resistant derivative R-U937. Bisulphite-converted DNA from the samples were hybridised to Illumina HumanMethylation450 BeadChips.
Project description:array-based analysis of genome-wide DNA methylation changes induced by the demethylating drugs 5-azacytidine and CP-4200 on U937 cells for 72 hours
Project description:ABL cells (derivative of LNCaP) were treated with 100µM UK5099 or vehicle (control) for 72 hours, at which time total RNA was collected and analyzed using RNA-sequencing
Project description:The following study was conducted to better understand the genetic response of maize roots to nitrate availability. To do so, an array was designed and created by the Plant Sciences Institute at Iowa State University. This custom array contained 7,888 informative spots of cDNA obtained from NSF Plant Genome EST projects led by Ginny Walbot and Pat Schnable. RNA was collected from root tissue at two time points: 30 minutes and 24 hours. At each time point, transcript profiles were compared between root tissues treated with 5mM calcium nitrate and controls treated with 5mM calcium sulfate. Keywords: Nutrient response in maize roots
Project description:Transcriptional profiling of U937 scramble vs shHDAC2 before and after SAHA treatment at 5µM concentration for 6 and 24 hours. Different Experimental Conditions: U937 scramble (U937 trasfected with empty vector) vs shHDAC2 (U937 trasfected with shHDAC2 vector), untreated and treated with SAHA at 5 µM concentration for 6h and 24h. Biological replicates: 2 for each sample, independently grown and harvested at 6 and 24 hours. One replicate per array.
Project description:U937 AML cells that express an inducible AML1-ETO construct under the control of the tetracycline promoter. Microarrays used to discover an AML1-ETO signature for a GE-HTS screen to identify AML1-ETO modulators. Experiment Overall Design: U937 AML cells that express an inducible AML1-ETO construct under the control of the tetracycline promoter at 0, 12, 24, 48, and 72 hours and corresponding controls (2 replicates each).
Project description:3T3-L1 preadipocytes were treated with control siRNA and two siRNA to FTO: siFTO-1 and siFTO-2. 24 hours after transfection, cells were induced to differentiation with MDI cocktail. RNA were collected from non-treated cells, and those treated with control siRNA, siFTO-1 or siFTO-2 at 0, 6, 24 and 72 hours post induction. Three replicates were done for each data point.
Project description:Expression profiles of one-week-old rice seedlings exposed to Pi-deficient solution for 6, 24, 48, 72 hours were monitored by microarray that contains approximately 60,000 rice clones (Affymetrix GeneChip). The probes were prepared from RNAs isolated from rice seedlings exposed to Pi-deficient solution for 6, 24, 48, 72 hours, respectively, and those non-treated controls. For hybridization, two biological replicates were used to extract RNAs from different batches of plants.
