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Opitutus terrae PB90-1

ABSTRACT: Rice paddy soil bacterium

PROVIDER: PRJNA19989 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR001578.fastq.gz	Fastqsanger.gz
	SRR001579.fastq.gz	Fastqsanger.gz
	SRR9434820.fastq.gz	Fastqsanger.gz
	SRR9434819.fastq.gz	Fastqsanger.gz

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Project description:We present the draft genome of Nitrospirae bacterium Nbg-4 as a representative of this clade and couple this to in situ protein expression under sulfate-enriched and sulfate-depleted conditions in rice paddy soil. The proteins were extracted from the soil and analysed via LC-MS/MS measurements.

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Project description:Bacillus velezensis strain GH1-13 isolated from a rice paddy soil in Korea has been reported to promote plant growth and inhibit some pathogens. It contains a plasmid pBV71, thought to be of benefit to the strain, but there is no information on its effect. In order to elicit the plasmid effect on gene expression, mRNA and protein levels were analyzed at various stages of bacterial growth. Comparative gene expression profiles between the plasmid-containing and plasmid-free cells revealed that strain GH1-13 activated a transient stress response in the exponential phase. It showed early activation of expression of sigma W operon, liaIHGFSR operon, and transcription regulators for transition state, associated with carbon catabolite repression and secondary metabolite biosynthesis of acetoin, bacillaene, and macrolactin.

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