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Small RNA-seq of human granulosa cells reveals miRNAs in FSHR and aromatase genes

ABSTRACT: Small RNA-seq of human granulosa cells reveals miRNAs in FSHR and aromatase genes

OTHER RELATED OMICS DATASETS IN: E-GEOD-46490

PROVIDER: PRJNA200700 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR836166.fastq.gz	Fastqsanger.gz
	SRR836167.fastq.gz	Fastqsanger.gz
	SRR836168.fastq.gz	Fastqsanger.gz
	SRR836169.fastq.gz	Fastqsanger.gz
	SRR836170.fastq.gz	Fastqsanger.gz

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MicroRNAs expressed from FSHR and aromatase genes target important ovarian functions.

Project description:MicroRNAs (miRNAs) have known roles in the post-transcriptional regulation of various biological processes including ovarian follicle development. We have previously identified from human pre-ovulatory granulosa cells miRNAs that are expressed from the intronic regions of two key genes in normal follicular development: FSH receptor (FSHR) and CYP19A1, the latter encoding the aromatase enzyme. In the present study, we aim to identify the targets regulated by those two miRNAs: hsa-miR-548ba and hsa-miR-7973, respectively. The miRNAs of interest were endogenously expressed in KGN cell-line, gene expression changes were analyzed by Affymetrix microarray and confirmed by RT-qPCR. Potential miRNA-regulated sequences were further filtered from the obtained results by bioinformatic target prediction algorithms and validated for direct miRNA:mRNA binding by luciferase reporter assay. Our results verified Leukemia inhibitory factor receptor (LIFR), Phosphatase and tensin homolog (PTEN), Neogenin 1 (NEO1) and SP110 nuclear body protein (SP110) as target genes for hsa-miR-548ba. Hsa-miR-7973 target genes ADAM metallopeptidase domain 19 (ADAM19), Peroxidasin (PXDN) and Formin like 3 (FMNL3) also passed all verification steps. In conclusion we propose that hsa-miR-548ba may be involved in the regulation of follicle growth and activation via LIFR and PTEN. Hsa-miR-7973 may be implicated in the modulation of extracellular matrix and cell-cell interactions. Taken together, our results suggest that those two miRNAs of interest have important regulatory roles in granulosa cells and in follicle development in general.

2020-01-30 | GSE122731 | GEO

Research resource: small RNA-seq of human granulosa cells reveals miRNAs in FSHR and aromatase genes.

Project description:The granulosa cells in the mammalian ovarian follicle respond to gonadotropin signaling and are involved in the processes of folliculogenesis and oocyte maturation. Studies on gene expression and regulation in human granulosa cells are of interest due to their potential for estimating the oocyte viability and in vitro fertilization success. However, the posttranscriptional gene expression studies on micro-RNA (miRNA) level in the human ovary have been scarce. The current study determined the miRNA profile by deep sequencing of the 2 intrafollicular somatic cell types: mural and cumulus granulosa cells (MGCs and CGCs, respectively) isolated from women undergoing controlled ovarian stimulation and in vitro fertilization. Altogether, 936 annotated and 9 novel miRNAs were identified. Ninety of the annotated miRNAs were differentially expressed between MGCs and CGCs. Bioinformatic prediction revealed that TGFβ, ErbB signaling, and heparan sulfate biosynthesis were targeted by miRNAs in both granulosa cell populations, whereas extracellular matrix remodeling, Wnt, and neurotrophin signaling pathways were enriched among miRNA targets in MGCs. Two of the nine novel miRNAs found were of intronic origin: one from the aromatase and the other from the FSH receptor gene. The latter miRNA was predicted to target the activin signaling pathway. In addition to revealing the genome-wide miRNA signature in human granulosa cells, our results suggest that posttranscriptional regulation of gene expression by miRNAs could play an important role in the modification of gonadotropin signaling. miRNA expression studies could therefore lead to new prognostic markers in assisted reproductive technologies.

| S-EPMC5415242 | biostudies-literature

MicroRNAs expressed from FSHR and aromatase genes target important ovarian functions.

Project description:MicroRNAs expressed from FSHR and aromatase genes target important ovarian functions.

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Differentially expressed miRNAs in granulosa cell of diminished ovarian reserve (DOR) patients

Project description:To investigate the pathogenesis of diminished ovarian reserve, differentially expressed miRNAs in granulosa cells were constructed through miRCURYTM LNA expression Array.

2018-03-14 | GSE111771 | GEO

Small RNA-seq of human granulosa cells reveals miRNAs in FSHR and aromatase genes

Project description:This SuperSeries is composed of the SubSeries listed below.

2013-08-05 | GSE46508 | GEO

Small RNA-seq of human granulosa cells reveals miRNAs in FSHR and aromatase genes

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-08-05 | E-GEOD-46508 | biostudies-arrayexpress

Expression profile of miRNAs in Extracellular vesicles (EVs) obtained from Bovine granulosa cells subjected to heats stress

Project description:The study aimed to uncover the release of miRNAs via EVs and the differential release of these miRNAs from bovine granulosa cells in response to heat stress

2020-09-30 | GSE146491 | GEO

DENND1A Desensitizes Granulosa Cells to FSH by Arresting Intracellular FSHR Transportation

Project description:Genome-wide association studies (GWAS) have identified polycystic ovary syndrome (PCOS)-associated loci, including DENND1A encoding a clathrin-binding protein involved in vesicle transport as a guanine nucleotide exchange factor. The effect of DENND1A on reproduction and whether it participates in follicle development disorder and androgen synthesis in PCOS remain unknown. Here, we demonstrated that DENND1A expression increased in ovarian granulosa cells (GCs) of PCOS patients and was positively correlated with serum testosterone concentration. We constructed a systemic transgenic Dennd1a mouse (TG mouse) model, which showed subfertility, irregular estrous cycles, and increased production of testosterone after PMSG stimulation. Moreover, TG mice showed hyporesponsiveness to PMSG with smaller ovary size and less well-developed follicles. Intracellular follicle-stimulating hormone receptor (FSHR) transport was disturbed by overexpression of Dennd1a, which promoted FSHR internalization and inhibited its recycling. Our findings revealed the reproductive function of DENND1A and the underlying mechanisms, which provided new insights into the PCOS pathogenesis and contributing to drug design for PCOS treatment.

2023-07-05 | GSE236248 | GEO

DENND1A Desensitizes Granulosa Cells to FSH by Arresting Intracellular FSHR Transportation

Project description:DENND1A Desensitizes Granulosa Cells to FSH by Arresting Intracellular FSHR Transportation

| PRJNA989568 | ENA

Differentially expressed miRNAs in granulosa cell of diminished ovarian reserve (DOR) patients

Project description:Differentially expressed miRNAs in granulosa cell of diminished ovarian reserve (DOR) patients

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