Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA203259 | ENA |
REPOSITORIES: ENA
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Project description:There is great interest in substituting animal with in vitro experimentation in human health risk assessment, but there are rather few comparisons of in vitro and in vivo biological responses to engineered nanomaterials (ENM). We used high-content genomics tools, to compare in vivo pulmonary responses of multiwalled carbon nanotubes (MWCNT) to those in vitro in cultured lung epithelial cells at the global transcriptomic level. Mice were exposed via a single intratracheal instillation to 18, 54 or 162 ?g of Mitsui7 and samples were collected at 24 hours post-instillation. This experiment examined the pulmonary transcriptional response of female C57BL/6 mice exposed to Mitsui7 multiwalled carbon nanotubes at three doses: D1 (18 ?g), D2 (54 ?g), D3 (162 ?g), and vehicle control. Each dose group was examined 24 hours post-exposure. Each dose group had 6 biological replicates. There were a total of 22 samples included in the final analysis using a two-color reference design.
2015-05-05 | E-GEOD-46998 | biostudies-arrayexpress
Project description:There is great interest in substituting animal with in vitro experimentation in human health risk assessment, but there are rather few comparisons of in vitro and in vivo biological responses to engineered nanomaterials (ENM). We used high-content genomics tools, to compare in vivo pulmonary responses of multiwalled carbon nanotubes (MWCNT) to those in vitro in cultured lung epithelial cells at the global transcriptomic level. Mouse lung epithelial cells were incubated with 12.5, 25 and 100 μg/ml of Mitsui7 and harvested at 24 hours post-exposure. This experiment examined the mouse lung epithelial cell line FE1's response following exposure to Mitsui7 multiwalled carbon nanotubes at three doses: D1 (12.5 μg/ml), D2 (25 μg/ml), D3 (100 μg/ml), and vehicle control. Each dose group was examined 24 hours post-exposure. Each dose group had 6 biological replicates. There were a total of 22 samples included in the final analysis using a two-color reference design.
2015-05-05 | E-GEOD-46999 | biostudies-arrayexpress
Project description:Transcriptomic analysis of mouse lung tissue exposed to multiwalled carbon nanotubes (Mitsui7)
| PRJNA203260 | ENA
Project description:There is great interest in substituting animal with in vitro experimentation in human health risk assessment, but there are rather few comparisons of in vitro and in vivo biological responses to engineered nanomaterials (ENM). We used high-content genomics tools, to compare in vivo pulmonary responses of multiwalled carbon nanotubes (MWCNT) to those in vitro in cultured lung epithelial cells at the global transcriptomic level. Mice were exposed via a single intratracheal instillation to 18, 54 or 162 μg of Mitsui7 and samples were collected at 24 hours post-instillation.
2015-05-05 | GSE46998 | GEO
Project description:There is great interest in substituting animal with in vitro experimentation in human health risk assessment, but there are rather few comparisons of in vitro and in vivo biological responses to engineered nanomaterials (ENM). We used high-content genomics tools, to compare in vivo pulmonary responses of multiwalled carbon nanotubes (MWCNT) to those in vitro in cultured lung epithelial cells at the global transcriptomic level. Mouse lung epithelial cells were incubated with 12.5, 25 and 100 μg/ml of Mitsui7 and harvested at 24 hours post-exposure.
2015-05-05 | GSE46999 | GEO
Project description:Expression profiling of mice exposed to multiwalled carbon nanotubes
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Project description:Transcriptomic analysis of mouse lung tissue exposed to two multiwalled carbon nanotubes (NRCWE-26 and NM-401)
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Project description:In vivo pulmonary genotoxicity of multiwalled-carbon nanotubes
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Project description:Mice were aspirated with multiwalled carbon nanotubes in four doses ranging from 10 µg to 80 µg, plus control, and sacrificed after 1, 7, 28, and 56 days. RNA was extracted from the lungs and expression profilling by microarray was performed. This is a reference design two-color experiment. Each array was hybridized with RNA extracted from mouse lung labeled with Cy3, and universal reference RNA labeled with Cy5. Five different doses (0, 10ug, 20ug, 40ug, and 80ug) of multiwalled carbon nanotube aspiration were used and mice were sacrificed after 1, 7, 28, and 56 days. Eight replicates were used at each time/dose condition for a total of 160 arrays.
2012-06-25 | E-GEOD-29042 | biostudies-arrayexpress
Project description:Mice were aspirated with multiwalled carbon nanotubes in four doses ranging from 10 µg to 80 µg, plus control, and sacrificed after 1, 7, 28, and 56 days. RNA was extracted from the lungs and expression profilling by microarray was performed.
2012-06-26 | GSE29042 | GEO