Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

In Vivo Endothelial Cell Heterogeneity

ABSTRACT: In Vivo Endothelial Cell Heterogeneity

PROVIDER: PRJNA203650 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Human Organ-Specific Endothelial Cell Heterogeneity

Project description:Human Organ-Specific Endothelial Cell Heterogeneity

| PRJNA471906 | ENA

In Vivo Endothelial Cell Heterogeneity

Project description:Endothelial cells from nine steady state tissues and two regenerating tissues (bone marrow and liver) were intravitally labeld, isolated via flow sorting, and immediately processed for RNA extraction. When of sufficient quality, the RNA was amplified and hybridized. For comparison, Human Emybryonic Stem Cell-derived Endothelial cells (hESC-ECs) were differentiated and isolated based on similarities to the adult mouse counterparts. Endothelial cells were labeled via intravitally labeling of the vascular bed 8 minutes prior to sacrifice with minimally three markers to identify endothelial cells followed by flow sorting.

2013-07-15 | E-GEOD-47067 | biostudies-arrayexpress

In Vivo Endothelial Cell Heterogeneity

2013-07-15 | GSE47067 | GEO

Single-cell transcriptome Atlas reveals embryonic endothelial heterogeneity

Project description:Single-cell transcriptome Atlas reveals embryonic endothelial heterogeneity

| PRJNA896490 | ENA

Microvascular endothelial heterogeneity

Project description:The goal of this study was to gain insight into the molecular heterogeneity of capillary endothelial cells derived from different organs by microarray profiling of freshly isolated cells and identify transcription factors that may determine the specific gene expression profile of endothelial cells from different tissues. The study focused on heart endothelial cells and presents a validated signature of 31 genes that are highly enriched in heart endothelial cells. Within this signature 5 transcription factors were identified and the optimal combination of these transcription factors was determined for specification of the heart endothelial fingerprint. From three tissue types (mouse brain, heart and liver), we collected five freshly isolated endothelial cell samples each. For each brain sample we pooled RNA from 6 mice. For each heart sample we pooled RNA from 4 mice. For each liver sample we pooled RNA from 2 mice. The three endothelial subtypes were then compared. For each subtype, specific gene profiles were defined by determining the genes that were highly enriched versus the other two endothelial subtypes.

2015-02-19 | E-GEOD-48209 | biostudies-arrayexpress

Single-cell transcriptome analysis of endothelial cell heterogeneity under laminar shear stress

Project description:Heterogeneity of endothelial cells that form the innermost layer of all vessels is critical for vascular sprouting and angiogenesis. After new vessels form, endothelial cell heterogeneity is believed to be gradually lost as vessels respond to flow-mediated signals, mature, remodel and become homeostatic. However, whether and at what level endothelial cell lost heterogeneity is poorly understood. Here we investigated heterogeneity change of endothelial cells under homeostatic laminar shear tress in comparison to static cultures.

2021-05-25 | GSE151867 | GEO

MERFISH reveals embryonic endothelial heterogeneity

Project description:MERFISH reveals embryonic endothelial heterogeneity

| PRJNA1038713 | ENA

In vivo endothelial transcriptional profiles of porcine coronary and iliac arteries

Project description:Phenotypic heterogeneity among arterial ECs is particularly relevant to atherosclerosis since the disease occurs predominantly in major arteries, which vary in their atherosusceptibility. To explore EC heterogeneity, we used DNA microarrays to compare gene expression profiles of freshly harvested porcine coronary and iliac artery ECs. We demonstrate that in vivo the endothelial transcriptional profile of a coronary artery (the right coronary artery) is intrinsically different from that of a major conduit vessel (the external iliac artery), and that this difference is consistent with former vessel being more prone to atherosclerosis. Keywords: coronary atherosclerosis, endothelial heterogeneity, microarray, gene expression Endothelial cells were freshly harvest from right coronary, left and right iliac arteries from four pigs. RNA were isolated and expression profiles were obtained using olig microarrays.

2010-06-20 | E-GEOD-10938 | biostudies-arrayexpress

Loss of endothelial cell heterogeneity in arteries after obesogenic diet

Project description:Loss of endothelial cell heterogeneity in arteries after obesogenic diet

| PRJNA984738 | ENA

Single-cell transcriptome analysis of endothelial cell heterogeneity under laminar shear stress

Project description:Single-cell transcriptome analysis of endothelial cell heterogeneity under laminar shear stress

| PRJNA637536 | ENA