Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens strain:HeLa cell line

ABSTRACT: Genome-wide locatlization analyses of RXR binding sites in the presence or absence of LG268 in HeLa cell line.

PROVIDER: PRJNA208288 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR907525.fastq.gz	Fastqsanger.gz

Items per page:

1 - 1 of 1

Similar Datasets

Characterisation of the AGO2 binding sites in HeLa cell line using eiCLIP

Project description:Characterisation of the AGO2 binding sites in HeLa cell line using eiCLIP

| PRJEB62149 | ENA

Characterisation of the AGO2 binding sites in HeLa cell line using eiCLIP

Project description:Characterisation of the AGO2 binding sites in HeLa cell line using eiCLIP

| PRJEB62152 | ENA

Genome-wide TNFM-NM-1-induced p65 binding before and after telomerase inhibition in HeLa cells

Project description:Chromatin immunoprecipitation sequencing (ChIP-seq) was performed to analyze the effect of telomerase inhibition on TNFM-NM-1-induced genome-wide p65 binding in HeLa cells. By obtaining over 40 million uniquely mappable reads per sample from ChIP-seq, maps for TNFM-NM-1-induced p65 binding in absence and presence of an hTERT inhibitor, MST-312, were generated. As expected, TNFM-NM-1 treatment significantly increased genome-wide p65 occupancy. Interestingly, when cells were treated with MST-312 prior to TNFM-NM-1 stimulation, the number of p65 binding sites was reduced. In addition, some binding sites, including important p65 targets like IL6 and TNF, showed a reduced p65 occupancy with a minimum fold change of 1.5, after MST-312 exposure. Taken together, our ChIP-seq data indicate that telomerase is required for optimal p65 binding at a small proportion of p65 target sites upon inflammatory stimuli. Examination of p65 binding in HeLa cells in absence and presence of TNFM-NM-1 and MST-312.

2012-11-26 | E-GEOD-41100 | biostudies-arrayexpress

The effect of Neuroserpin expression upon HeLa cell gene expression

Project description:HeLa cells were stably transfected using the Tet-On® Advanced Cell Line system with Wildtype, G392E and Delta Neuroserpin with neuroserpin expression induced with doxycycline. Gene expression was examined in the absence or presence of 2 ug/ml doxycycline.

2013-04-20 | GSE46230 | GEO

Genome-wide TNFα-induced p65 binding before and after telomerase inhibition in HeLa cells

Project description:Chromatin immunoprecipitation sequencing (ChIP-seq) was performed to analyze the effect of telomerase inhibition on TNFα-induced genome-wide p65 binding in HeLa cells. By obtaining over 40 million uniquely mappable reads per sample from ChIP-seq, maps for TNFα-induced p65 binding in absence and presence of an hTERT inhibitor, MST-312, were generated. As expected, TNFα treatment significantly increased genome-wide p65 occupancy. Interestingly, when cells were treated with MST-312 prior to TNFα stimulation, the number of p65 binding sites was reduced. In addition, some binding sites, including important p65 targets like IL6 and TNF, showed a reduced p65 occupancy with a minimum fold change of 1.5, after MST-312 exposure. Taken together, our ChIP-seq data indicate that telomerase is required for optimal p65 binding at a small proportion of p65 target sites upon inflammatory stimuli.

2012-11-26 | GSE41100 | GEO

Site-specific N-glycosylation of HeLa cell glycoproteins

Project description:HeLa cell line is frequently used in biomedical research, however little is known about N-glycan structures expressed on individual glycoproteins of this complex sample. We characterized site-specific N-glycosylation of HeLa N-glycoproteins using a complex workflow based on high and low energy tandem mass spectrometry experiments and rigorous data evaluation. The analyses revealed high amount of bovine serum contaminants compromising previous results focusing on released glycan analysis. We reliably identified 43 (human) glycoproteins, 69 N-glycosylation sites and 178 glycopeptides following an acetone precipitation based sample enrichment step. HeLa glycoproteins were found to be highly fucosylated and in several cases localization of the fucose (core or antenna) could also be determined based on low energy tandem mass spectra. High-mannose sugars were expressed in high amounts as expected in case of a cancer cell line. Our method enabled the detailed characterization of site-specific N-glycosylation of several glycoproteins expressed in HeLa. Furthermore, we were the first to experimentally prove the existence of 31 glycosylation sites, where previously presence of glycosylation was only predicted based on the existence of the consensus sequon.

2019-10-21 | PXD013930 | Pride

Homo sapiens cultivar:HeLa cell line

Project description:Homo sapiens cultivar:HeLa cell line Raw sequence reads

| PRJNA435660 | ENA

EWS siRNA knock-down in HeLa cell line

Project description:HeLa cell line was trasfected by a EWS siRNA oligo causing knock-down of EWS or a siRNA scrambled oligo

2011-09-20 | GSE22929 | GEO

MYC binding sites in HeLa cells

Project description:MYC binding sites determined in HeLa cells using ChIP-chip Keywords: ChIP-chip

2006-03-09 | GSE4354 | GEO

Characterization of MGC-803 cell line use sample HeLa and AGS

Project description:Investigation of the mixed origins of the MGC-803 cell line reveals it is a HeLa hybrid cell line