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Omics score: 0

Mus musculus

ABSTRACT: Runx3 function in IL-2-activated splenic CD8+ T cells.

OTHER RELATED OMICS DATASETS IN: E-GEOD-50121E-GEOD-50122E-GEOD-50123E-GEOD-50119

PROVIDER: PRJNA216117 | ENA |

REPOSITORIES: ENA

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Genome-wide maps of Runx3 bound regions in splenic IL-2-activated CD8+ T cells

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Project description:Cellular binary fate decisions require the progeny to silence genes associated with the alternative fate. The major subsets of alpha:beta T cells have been extensively studied as a model system for fate decisions. While the transcription factor RUNX3 is required for the initiation of Cd4 silencing in CD8 T cell progenitors, it is not required to maintain the silencing of Cd4 and other helper T lineage genes. The other runt domain containing protein, RUNX1, silences Cd4 in an earlier T cell progenitor, but this silencing is reversed whereas the gene silencing after RUNX3 expression is not reverse. Therefore, we hypothesized that RUNX3 and not RUNX1 recruits other factors that maintains the silencing of helper T lineage genes in CD8 T cells. To this end, we performed a proteomics screen of RUNX1 and RUNX3 to determine candidate silencing factors.

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