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E-GEOD-50122

ABSTRACT: Runx3 function in splenic NK cells (IL-2 or IL-15)

PROVIDER: E-GEOD-50122 | ExpressionAtlas | 2015-07-13

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-50122-analysis-methods.tsv	Tabular
	E-GEOD-50122-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-50122-configuration.xml	Xml
	E-GEOD-50122-percentile-ranks.tsv	Tabular
	E-GEOD-50122.condensed-sdrf.tsv	Tabular

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Genome-wide maps of Runx3 bound regions in splenic NK cells activated in vivo with IL-15

Project description:ChIP-seq was conducted using freshly isolated splenic WT NK cells from IL-15/Ra treated mice with anti-Runx3 antibody (Ab) and non-immune serum (NIS) as control. Runx3 and NIS IP from splenic NK cells of IL-15/Ra treated WT mice, isolated by negative selection using NK cell isolation kit (R&D) followed by sorting of NKp46+ cells.

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Project description:ChIP-seq was conducted using splenic WT NK cells cultured for 7 days with IL-2 using anti-Runx3 antibodies (Ab), anti-H3K4me1 Ab and non-immune serum (NIS) as control. Two biological Runx3, one H3K4me1 and two NIS IP repeats from splenic NK cells isolated from individual mice by negative selection using NK cell isolation kit (R&D) cultured with IL-2.

2013-11-18 | E-GEOD-50129 | biostudies-arrayexpress

Genome-wide maps of Runx3 bound regions in splenic NK cells activated in vivo with IL-15

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E-GEOD-50120

Project description:Runx3 function in splenic NK cells activated in vivo by IL-15.

2015-07-13 | E-GEOD-50120 | ExpressionAtlas

Runx3 function in splenic NK cells (IL-2 or IL-15)

Project description:NK cells are innate immune cells that recognize and kill foreign, virally-infected and tumor cells without the need for prior immunization. NK expansion following viral infection is IL-2 or IL-15-dependent. To identify Runx3 responsive genes, NK cells were isolated from spleen of WT and Runx3-/- mice . Ten samples (5 WT and 5 Runx3-/-) of freshly isolated NK cells were separately obtained from individual mice. Cells were cultured for 7 days with IL-2 or IL-15.

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Genome-wide maps of Runx3 bound regions in splenic NK cells

Project description:ChIP-seq was conducted using freshly isolated (resting) splenic WT NK cells with anti-Runx3 antibody (Ab), anti-H3K4me1 Ab and non-immune serum (NIS) as control. Runx3 and H3K4me1 IP from splenic NK cells isolated by negative selection using NK cell isolation kit (R&D) followed by sorting of NKp46+ cells.

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Runx3 function in splenic NK cells

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2013-11-18 | E-GEOD-50123 | biostudies-arrayexpress

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