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Francisella tularensis subsp. tularensis str. SCHU S4 substr. FTS-634/635

ABSTRACT: Francisella tularensis subsp. tularensis str. SCHU S4 substr. FTS-634/635 Genome sequencing and assembly

PROVIDER: PRJNA217353 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR1061346_1.fastq.gz	Fastqsanger.gz
	SRR1061346_2.fastq.gz	Fastqsanger.gz
	SRR999319_1.fastq.gz	Fastqsanger.gz
	SRR999319_2.fastq.gz	Fastqsanger.gz

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Project description:Differential expression in human peripheral blood monocytes between F. novicida-infected and uninfected, and between Francisella tularensis tularensis isolate Schu S4 and uninfected. The goal was to examine genomewide transcriptional reponses to these two strains, and identify differentially-regulated genes that may help explain the virulence of Schu S4. Experiment Overall Design: Human monocytes were infected with the Schu S4 isolate of Francisella tularensis tularensis (n=4), with F. tularensis subspecies novicida isolate U112 (n=4) or were left uninfected (n=6). Gene expression values were calculated using the gcrma package in R and BioConductor, and limma to identify differentially expressed genes. Submitted here are expression values calculated using R 2.7.1 and BioConductor 2.2 (FreeBSD/amd64) but the original were done using R 2.6.1 and BioConductor 2.1 (FreeBSD/amd64). Twelve other chips were pooled with these 14 for preprocessing.

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